Fig 1: Ferrostatin-1 (Fer-1) alleviates ferroptosis in corpus cavernosum smooth muscle cells (CCSMCs): (A) the results of the Cell Counting Kit-8 (CCK-8) assay; (B) representative Western blot results for GPX4, SLC7A11, ACSL4, and ALOX15; (C–F) relative expression of GPX4, SLC7A11, ACSL4, and ALOX15 compared with ß-actin; data are shown as the fold changes over the control group; n = 9 for each group; (G) malondialdehyde (MDA) content results; (H) the results of superoxide dismutase (SOD) activity; (I) relative total iron content, n = 6 for each group; and (J) representative results of reactive oxygen species (ROS). *p < 0.05 compared with the control group. # p < 0.05 compared with the high glucose (HG) group
Fig 2: The expression level of GPX4, ACSL4, and SLC7A11: (A) representative results of immunohistochemistry (IHC) and relative expression of GPX4; (B) representative results of IHC and relative expression of ACSL4; and (C) representative results of IHC and relative expression of SLC7A11. *p < 0.05 compared with the control group
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