Fig 1: Expression of key proteins of necroptosis in HUVECs.a–c TNFR, TRADD (a), caspase-8 and FADD (b) mRNA and their protein expressions (c) in co-cultured HUVECs were validated by qRT-PCR and western blotting analysis, respectively. d–f NF-?B (d) and p38 MAPK mRNA (e) and their protein expressions (f) in co-culured HUVECs were detected by qRT-PCR and western blotting analysis, respectively. g The proposed model of HPSE/SDC-1/TNF-a axis and p38 MAPK pathway in HPSE induced necroptosis. *P < 0.05, **P < 0.01, and ***P < 0.001.
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