Fig 1: TP-induced senescence partly modulates sarcoplasmic reticulum-related proteins. (A-D) Western blot and quantification analyses of PLB, SERCA2a and RYR2 expression in human samples (n=8-12). (E-H) The protein expression of PLB, SERCA2a and RYR2 was determined by Western blot analysis in control nonpaced (0 HZ; CTRL), normal-paced (1 HZ) and TP (5 HZ) cells for 12 h. GAPDH was used as a loading control (n=3). (I-K) mRNA expression of PLB, SERCA2a and RYR2 after treatment with siNC TP or si-p21 TP for 12 hours (n=3). (L-O) Protein expression and quantification analyses of PLB, SERCA2a and RYR2 after treatment with CTRL, siNC TP or si-p21 TP for 12 hours (n=3-6). Comparisons were performed using one-way ANOVA for three groups and Student’s t-test for two groups.