Fig 1: Phenotypic characterization of human adipose-derived MSCs. a The fibroblast-like morphology of adhesion cells was studied by phase-contrast microscopy. Scale 100 µm. b Multilineage differentiation of MSCs. Histochemical staining of calcium deposits (alizarin red) and neutral lipid accumulation (oil red O) after 14 days of osteogenesis and adipogenesis induction. Scale 100 µm. c Flow cytometry analysis of TLR3 expression: after 10 µg/mL poly(I:C) stimulation for 3 h – prMSCs (red line) and without stimulation – MSCs (constitutive expression, blue line), isotypic control—IC (green line). d, e Immunophenotypic characteristics of MSCs and prMSCs: negative expression of CD34, CD45, and HLA-DR markers (top panel); positive expression of CD73, CD90, and CD105 markers (bottom panel)
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