Fig 1: Transcriptional signatures of pre–pro B cells and committed pro-B cells.a Highlighted populations for pre–pro B and cycling pro-B cells (left). Feature plots for early B-cell markers (right). b Heatmap of genes that are differentially expressed between the pre–pro B cells and the cycling pro-B cells. Scale represents normalized counts centered and scaled across cells. c Feature plot of EBF1-target genes. d Differentially upregulated genes in the CD93 ADT high versus CD93 ADT low pre–pro B-cell cluster. CD93 high versus low was based on a median split. No genes were significantly downregulated in the CD93 high population. e Feature plot of genes denoting the pro-B-cell stage (Kit), or genes uniquely expressed during pro-B cells. f Violin plot of the expression of surrogate light chains, Vpreb1 and Igll1 across the B-cell development stages. Color scale in a, c, e, and f represents natural log-transformed SCTransform-corrected counts.