Fig 1: CD90+ SFs show a distinct expression of sialyltransferases in experimental arthritis. (A) SFs were isolated from joints of naïve and mice undergoing Collagen-Induced Arthritis. Cells were sorted by FACs gating on low viability dye, CD31-, CD45- and PDPN+ cells. Subsets of SFs were discriminated by expression of CD90. Sorting strategy is shown in the dot plots. (B) Total number of isolated cells from paws from naïve (n = 7) and CIA (n = 10) animals. (C) Relative proportions of SFs subset (lining: CD90- and sublining: CD90+) in total SFs of naïve (n = 11) and arthritic mice (n = 12) evaluated by flow cytometry. (D) Relative expression of IL-6, CCL2 and MMP3 mRNA in SFs subsets were quantified by RT-qPCR (n ≥ 6). (E) Relative expression of α2,3- and α2,6-sialyltransferases (St6gal1, St6GalNac5, St3gal1, St3gal2, St3gal3, St3gal4 and St3gal6) were quantified in SFs subsets by RT-PCR (n ≥ 6). Data are represented as mean ± SEM; each dot represents SFs from one individual mouse. **p < 0.01 by Mann-Whitney test in (B, C), *p < 0.05, **p < 0.01, ***p < 0.001 by Kruskal-Wallis test in (D, E).