Fig 2: GLUT4 protein expression in skeletal muscle of rats in each group (x ± s; note: compared with the control group, ∗P < 0.05; compared with the model group, #P < 0.05.

Fig 3: Administration of Fn1 inhibitor increased the AMP/ATP ratio and activated LKB1-AMPK signaling in I/R hearts. (a) Immunoblotting analysis of the protein levels of p-LKB1 (S428), LKB1, p-AMKP (T172), AS160, GLUT4, Mfn1, Mfn2, (left), p-Drp1 (S616), and Drp1. GAPDH as an internal control. (b) The quantification of the relative protein levels (right, n = 4 per group). (c) Measurement of AMP and ATP contents and the ratio of AMP/ATP (n = 5). Data are expressed as mean ± SEM. ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001 versus the sham-operated group; ##P < 0.01; ###P < 0.001, ####P < 0.0001 versus saline I/R 24 h; &&P < 0.01; &&&P < 0.001, &&&&P < 0.0001 versus saline I/R 72 h.

Fig 4: DNM3OS regulated several pathways via promotion of GLUT4 and CD36 expression.A Hierarchical clustering of coding genes altered after knockdown of DNM3OS in GIST-882 cells. B KEGG analysis of deregulated genes in GIST-882-shDNM3OS cells. C Western blot assay showing the expression of GLUT4 and CD36 in GIST-882 cells and GIST-T1 cells. GAPDH was used as the loading control. D Protein–protein interaction analysis of DNM3OS-regulated proteins that are linked by GLUT4 and CD36. GIST gastrointestinal stromal tumor, KEGG Kyoto Encyclopedia of Genes and Genomes.

Fig 5: Validation of the prognostic value of metabolism-related risk model (MRM) in two independent prostate cancer (PCa) cohorts and real-world study. (A) Kaplan–Meier analysis for disease-free survival (DFS) curves of patients in low or high metabolism-related risk score (MRS) subgroups from two independent validation cohorts (Taylor and International Cancer Genome Consortium (ICGC) PCa). (B) Receiver operating characteristic (ROC) curves for predicting 1-, 3-, and 5-year DFS in patients from Taylor and ICGC PCa cohorts. (C) ROC analysis showed that the predictive accuracy of MRM in DFS was superior to other clinical features in Taylor and ICGC PCa cohorts. (D, E) Univariate and multivariate Cox regression analyses of MRS and clinical features in Taylor and ICGC PCa cohorts. (F, G) Immunohistochemistry (IHC) staining was performed to detect the key metabolism-related differentially expressed genes (mDEGs) (AHSG, APOE, BGN, SLC2A4, and CYP2D6) protein expression using PCa tissue arrays from 59 normal tissues and 196 tumor tissues. Representative images are shown. Statistical analysis of the immunoreactive score (IRS) scores of IHC staining. *p < 0.05; **p < 0.01; ***p < 0.001.