Fig 2: Placental ALLO insufficiency results in sex-linked cerebellar WM abnormalities at P30.a,b, Western blot analysis of myelin-related proteins in the male cerebellum. Data are presented as mean fold changes ± s.e.m. Multiple unpaired t-tests with Holm–Sidak multiple comparison test (*P < 0.05; ***P < 0.005). MBP: n = 12 C and 28 plKO (P = 0.038); MAG: n = 6 C and 6 plKO (P = 0.004); MOG: n = 6 C and 6plKO (P < 0.0001). c,d, Western blot analysis of myelin-related proteins in the female cerebellum. Data are presented as mean fold changes ± s.e.m. Multiple unpaired t-tests with Holm–Sidak multiple comparison test (*P < 0.05). MBP: n = 5 C and 4 plKO (P = 0.031); MAG: n = 5 C and 4 plKO (P = 0.18); MOG: n = 5 C and 4 plKO (P = 0.54). GAPDH, glyceraldehyde 3-phosphate dehydrogenase. e–h, Immunofluorescent staining of MBP in cerebellar lobule VII in males (e and f) and females (g and h). Scale bar, 150 µm. i, Scanning electron microscopy acquisition of a whole cerebellum ultrathin section showing the different cerebellar lobules (I–X) in a control mouse (×650 low magnification). The region of interest (inter-lobule VI–VII) for high-magnification acquisitions and g-ratio quantifications is indicated by the yellow rectangle. Scale bar, 300 µm. j, Schematic representation of an axon and its myelin sheath illustrating g-ratio. r, axon inner diameter; R, axon outer diameter. k–n, Representative scanning electron microscopy acquisitions (×20,000) of myelinated axons in inter-lobule VI–VII WM in males (k and l) and females (m and n). Scale bar, 300 nm. o, Scatter plot of g-ratios of >400 individual axons in males; data are from one representative control and one representative plKO mouse. Fitted lines are linear regressions. p, Percent of unmyelinated axons. Data are presented as mean ± s.e.m. Two-tailed unpaired Student’s t-test with Welch’s correction (*P < 0.05). n = 3 C and 3 plKO (P = 0.0275). q, Scatter plot of g-ratios of >400 individual axons in females; data are from one representative control and one representative plKO mouse. Fitted lines are linear regressions. r, Percent of unmyelinated axons. Data are presented as mean ± s.e.m. Two-tailed unpaired Student’s t-test with Welch’s correction (*P < 0.05). n = 3 C and 3 plKO (P = 0.0194). s, g-ratios by axon caliber category in males. Data are presented as mean ± s.e.m. Two-way ANOVA with Sidak’s multiple comparisons test (*P < 0.05). n = 3 C and 3 plKO (<1 µm: P = 0.0299; >1 µm: P = 0.0165). t, Axon caliber distribution in males (n = 3 C and 3 plKO). u, g-ratios by axon caliber category in females. Data are presented as mean ± s.e.m. Two-way ANOVA with Sidak’s multiple comparisons test (*P < 0.05). n = 3 C and 3 plKO <1 µm: P = 0.0162; >1 µm: P = 0.1185). v, Axon caliber distribution in females (n = 3 C and 3 plKO).Source data