Fig 1: A RBP4+NTS+ cancer cell subset is unique to left-sided CRC.(A) The t-SNE plot that showed the distribution of cancer cell lineages (yellow, n = 2196 cells) within the atlas. Cancer cell populations were reclustered into 9 subclusters (color coding). (B) Top 5 marker genes of 15 major cell types identified in this profile. (C) Annotation by left-sided and right-sided CRC cells. (D) The fraction of cells that originated from left-sided and right-sided CRC samples for 9 subgroups identified in this profile. (E) Kaplan-Meier survival curves of OS based on HSPA1A, GOLGB1, AGR3, MUC5AC, NTS, SPINK4, CLCA1, OLFM4, and PIGR expression using the online bioinformatics tool Kaplan-Meier Plotter. (F) Violin plots and immunochemistry display the distribution of expression of AGR2, AGR3, MUC5AC, SPINK4, TFF1, and TFF2 between left-sided and right-sided CRC. Scale bars: 100 µm.