Fig 1: Ligands of mGlu2/3 receptors influence expression of phosphorylated Fyn and subunits of N-methyl-D-aspartate receptors (NMDAR) in substantia nigra (SN) in sub-acute 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice. Western blot analysis showed the levels of p-GluN2A tyr1325 (A), p-GluN2B Tyr1472 (C) and p-Fyn Tyr416 (E) in SN of each group. Quantification of ratios of p-GluN2A tyr1325/GluN2A (B), p-GluN2B Tyr1472/GluN2B (D). *p < 0.05, **p < 0.01; #p < 0.05 vs. LY354740 + saline group. N = 3 per group in (B); N = 4 per group in (D). Quantification of the ratio of p-Fyn Tyr416/Fyn (F) of each group. *p < 0.05, **p < 0.01; #p < 0.05 vs. the normal + saline group. N = 3. Relative band intensities were calculated as a ratio of the phosphorylated protein to total protein. The data above were expressed as % of the normal + saline group and error bars represent SEM. Western blot analysis showed the levels of p-GluN2A tyr1325, p-Fyn Tyr416 (G) and p-GluN2B Tyr1472 (I) in SN of each group. (J) Quantification of ratios of p-GluN2A tyr1325/GluN2A and p-GluN2B Tyr1472/GluN2B of each group. *p < 0.05, **p < 0.01. N = 3. (H) Quantification of ratios of p-Fyn Tyr416/Fyn of each group. *p < 0.05; &&p < 0.01 vs. the other two groups. N = 3. Relative band intensities were calculated as a ratio of the phosphorylated protein to total protein. Data in (H, J) were expressed as % of the saline group and error bars represent SEM.