Fig 2: CBR-THC binding reduces invasive potential and disturbs mitochondrial dynamics in HTR8/SVneo cells. HTR8/SVneo cells were treated for 48 h as described in “Methods”, with DMSO and methanol (MeOH) used as vehicle controls. Summary histograms of relative MMP2 (A), MMP9 (B), TIMP2 (C), TIMP1 (D) MFN1 (E), MFN2 (F), OPA1 (G) and DRP1 (H) transcript expression in each treatment group normalized to 18S, then compared to the gene in the vehicle controls. Significant differences were determined by a one-way ANOVA, followed by a Bonferroni post hoc test. Data are presented as means ± SEM (n = 3). Different letters denote significant differences compared to vehicle controls (A–H, P < 0.0001). THC: 20 µM; AM281: 1 µM; AM630: 1 µM.

Fig 3: THC alters the expression of MMP and TIMP proteins in HTR8/SVneo cells. (A–D) Total protein was isolated from HTR8/SVneo cells and analyzed by Western blot (20 µg). Summary histograms of active MMP2 (A), pro-MMP9 and active MMP9 (B), TIMP1 (C), and TIMP2 (D) of relative band density in each treatment group normalized to stain-free image are shown below the Western blot images. Significant differences were determined by Student’s t-test. The individual points represent the ratios of proteins (n = 3), the horizontal lines represent the means and the error bars represent SEM. ** P < 0.01 (A,C), * P < 0.05 (B), **** P < 0.0001 (D).