Fig 1: Pharmacological actions of YJT via mediation of Sirt6/Nrf2/HO–1 signaling pathways. Mice were sacrificed and brain tissues were removed after the final LPS injection. The hippocampus was isolated from brain tissue and prepared either protein lysates (n = 6–9 to each group) or mRNA (n = 4–6 to each group) samples were prepared for further analysis. qPCR analysis was used to determine the mRNA expression levels of (A) Nox1 and (B) Nox2. Western blot analysis of (C) Sirt6, H3K56Ac, Total H3, Nrf2, HO–1, and Actinin were performed (n = 4 to each group). Finally, the gene expression level of (D) Sirt6 was quantified with qPCR. The data were expressed as mean ± SEM. ### p < 0.001 for Con vs. LPS, * p < 0.05, and ** p < 0.01 for LPS vs. drug–administrated groups.