Fig 1: Neutrophils from patients with CD40L deficiency show impaired migration.(A) Representative figure of gating strategy used to quantify migrated neutrophils (n = 7). (B) Neutrophils from patients with CD40L deficiency and healthy individuals (healthy control) were incubated with PBS, fMLP (20 nM), IL-8 (10 nM), or C5a (25 nM). Spontaneous migration (PBS) of healthy individuals was normalized to 100%, and the other conditions were compared with the normalized value to assess the relative migration number (expressed in %) (n = 7 [P1, P2, and P3 evaluated more than once]; C5a: n = 5). (C) Gating strategy and histograms showing the pattern of expression of FPR1, CXCR2, and C5aR receptors in CD66b+ neutrophils from patients with CD40L deficiency (colored) and healthy individuals (gray, dotted line). (D) Expression of FPR1, CXCR2, and C5aR receptors expressed in log2 obtained from median fluorescence intensity (MFI) values. n = 4. *P < 0.05 (Mann-Whitney U test).