Fig 1: Protein markers of murine CD45- cell subsets, related to Figure 3(A) CITE-seq data from the murine CD45- cells in Figure 3A were exported as an FCS file and an in silico gating strategy identified in FlowJo.(B) Gated cell overlay of populations identified using strategy in (A).(C) Expression of CD90, CD204, CD73, and CD29 markers by indicated cell types.(D) Expression of indicated protein markers in 60-plex MICS analysis in endothelial cells.(E) Expression of DESMIN, EPCAM, LYVE1, and CD31 at a portal triad (left) with inset (right).(F) Expression of indicated protein markers in 60-plex MICS analysis in stromal cells.(G) Molecular Cartography of indicated genes and cell types at portal vein. PV, portal vein; CV, central vein. Arrows indicate specific cell types, where color corresponds to cell type/markers. All images are representative of 2–6 mice.