Fig 1: Expression of the JUN gene and genomic redistribution of β-catenin, CBP, p300, and c-JUN (A) Expression values of JUN, JUNB, JUND, and FOS in the RNA-seq data. n = 3. (B) qRT-PCR results of the JUN gene. PANC-1 cells were treated with CHIR and ICG-001 or CHIR alone for the indicated times. JUN expression was normalized with ACTB and relative values to non-treated PANC-1 cells were graphed. n = 3. (C) The protein level of c-JUN at indicated times after CHIR-99021 + DMSO or CHIR-99021+ICG-001 treatment. The protein level of GAPDH was used as a reference for total protein load. Original images are provided as Fig2_JUN and Fig2_GAPDH in supplementary materials. (D) Venn diagram showing overlapping regions bound by c-JUN in cells cultured with or without ICG-001. (E–H) Peak intensities in β-catenin-associated regions with the TCF motif alone and regions with the TCF and AP-1 motifs. TCF motif alone; n = 191, TCF and AP-1 motifs; n = 156. Error bars represent the standard deviation. The comparison between cells cultured with or without ICG-001 at the same peaks was analyzed via paired Student's t-test. The comparison between regions with TCF motif only and regions with TCF and AP-1 motifs were analyzed using unpaired Student's t-test.