Fig 1: CD4+ TRM identification in cervix. a General gating strategy for phenotyping of CD4+ T cells obtained from cervicovaginal tissue of healthy donors. Gating strategy consisted of selecting hematopoietic CD45+ cells, followed by a double doublet exclusion, dead and CD19+ cells exclusion and finally a CD3+ CD4+ T cell gate from where CD69+/- cells were identified. b Representative flow cytometry plots of the expression of different cell-surface proteins and transcriptional factors in the CD4+CD69+/- T cell subsets from the cervical tissue of healthy donors (CD69- on the left column, CD69+ on the right column). c Frequency of different cell-surface proteins and transcriptional factors shown in b for CD4+CD69- T cells (empty circles) and CD4+CD69+ T cells (full circles; n = 5 for S1PR1; n = 6 for Eomes, T-bet, Hobit, CD49a; n = 7 for CCR7, PD-1). Lines and error bars represent median and interquartile ranges. Statistical comparisons were performed using Wilcoxon matched-pairs signed rank test to compare the two groups. Source data are provided as a Source Data file