Fig 2: Arc alleviates ICC injury. Distal colon segments were used for detection of ICC injury. Immunohistochemical localization of (A) C-Kit and (B) SCF in colon tissues. (C) Evaluation of immunohistochemical staining. (D) Immunoblotting for C-Kit and SCF. (E) Densitometry analysis of immunoblots. (F) Electron micrographs of the ICC in the colons of mice. The green arrows delineate the endoplasmic reticulum while the red arrows delineate the mitochondria. **P<0.01 vs. Control. ^P<0.05 and ^^P<0.01 vs. FC. FC, functional constipation; Arc, Arctiin; ICC, interstitial cells of Cajal; SCF, stem cell factor; C-Kit, receptor tyrosine kinase.

Fig 3: Representative HE and Azan Mallory staining from the H group. The images of MMP-9, MMP-2, TGFß1, and SCF immunostaining are also serial cross-sections from the same individual.