Purified anti-GAPDH 607901 from BioLegend

Product Specs
ItemPurified anti-GAPDH
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number607901
Quantity25 µg
ApplicationsWestern Blot (WB), Immunocytochemistry (ICC)
Concentration0.50 mg/mL
Conjugate/TagUnconjugated
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ReactivityHuman, Mouse
TargetGAPDH
IsotypeIgG2a, Kappa
CloneW17079A
HostRat
Antibody TypePrimary
ImmunogenRecombinant protein (161-335 a.a.)
NCBI Full Gene Nameprotein kinase C zeta
NCBI Gene AliasesPKC-ZETA, PKC2
ClonalityMonoclonal

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) ORF3c is expressed in SARS‐CoV‐2‐infected cells and inhibits innate sensing by targeting MAVSEMBO reportsDecember 6, 2023Martin Müller, Alexandra Herrmann, Shigeru Fujita, Keiya Uriu, Carolin Kruth, Adam Strange, Jan E Kolberg, Markus Schneider, Jumpei Ito, Marcel A Müller, et al.(1:1,000, Cell Signaling, Cat# 37829), rat polyclonal (IgG2a, κ) anti‐human GAPDH (1:1,000, BioLegend, Cat# 607902), rabbit polyclonal anti‐Sendai Virus (1:2,000, MBL Life science, Cat# PD029), rabbit monoclonal (IgG)

(2) Tetherin Restricts SARS-CoV-2 despite the Presence of Multiple Viral AntagonistsVirusesNovember 30, 2023Elena Hagelauer, Rishikesh Lotke, Dorota Kmiec, Dan Hu, Mirjam Hohner, Sophie Stopper, Rayhane Nchioua, Frank Kirchhoff, Daniel Sauter, Michael Schindleranti-V5 (Abcam, Cambridge, UK; ab9116), and monoclonal rat anti-GAPDH (BioLegend, San Diego, CA, USA; 607902). Conjugated antibodies: PE-conjugated human anti-BST2 (Miltenyi Biotec, Bergisch Gladbach, Germany;

(3) Endogenous IFITMs boost SARS-coronavirus 1 and 2 replication whereas overexpression inhibits infection by relocalizing ACE2iScienceApril 21, 2023Qinya Xie, Caterina Prelli Bozzo, Laura Eiben, Sabrina Noettger, Dorota Kmiec, Rayhane Nchioua, Daniela Niemeyer, Meta Volcic, Jung-Hyun Lee, Fabian Zech, et al.NL63,Ingenasa,M.30.HCo.I2D4 "Anti-Coronavirus Antibody, OC-43 strain",Millipore,Cat# MAB9012 Purified anti-GAPDH Antibody,Biolegend,Cat# 607902 Rabbit polyclonal anti-IFITM1,Cell signaling,Cat# 13126 S Rabbit polyclonal anti-IFITM2,Cell signaling,Cat#

(4) Cannabidiol and Cannabigerol Inhibit Cholangiocarcinoma Growth In Vitro via Divergent Cell Death PathwaysBiomoleculesJune 20, 2022Michael J. Viereckl, Kelsey Krutsinger, Aaron Apawu, Jian Gu, Bryana Cardona, Donovan Barratt, Yuyan Han(Danvers, MA, USA). Rat monoclonal antibody for Ki-67 (Cat #151202), rat monoclonal antibody GAPDH (Cat #607902), mouse monoclonal antibodies for β-actin (Cat #5714) and anti-mouse linked HRP goat polyclonal antibody

(5) SARS-CoV-2 Variants of Concern Hijack IFITM2 for Efficient Replication in Human Lung CellsJournal of VirologyJune 8, 2022Rayhane Nchioua et al.59212S), ACE2 (rabbit polyclonal) (Abcam, catalog no. ab166755), rat anti-GAPDH (Biolegend, catalog no. 607902), and SARS CoV-2 N (Sino Biologicals, catalog no. 40588-V08B) and infrared dye-labeled secondary antibodies

(6) Protein Disulfide Isomerase A3 Regulates Influenza Neuraminidase Activity and Influenza Burden in the LungInternational journal of molecular sciencesJanuary 19, 2022Chamberlain N, Ruban M, Mark ZF, Bruno SR, Kumar A, Chandrasekaran R, Souza De Lima D, Antos D, Nakada EM, Alcorn JF, et al.USA); PDIA6 (ab83456 Abcam, Waltham, MA, USA); β-actin (A5441 Sigma, St. Louis, MO, USA), and GAPDH (607902 Biolegend, San Diego, CA, USA) [3]. 4.14. Image Processing Digital images were acquired using an Amersham

(7) Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteinsSTAR protocolsDecember 17, 2021Hirschenberger M, Hayn M, Laliberté A, Koepke L, Kirchhoff F, Sparrer KMJ.,LI-COR Biosciences,Cat#926–68071; RRID:AB_10956166 "Monoclonal rat anti-GAPDH antibody, W17079A (1:1,000)",BioLegend,Cat#607902; RRID:AB_2734503 "Polyclonal rabbit anti-SARS-CoV-2 Nsp3 antibody (1:1,000)",GeneTex,Cat#GTX135614;

(8) Time-Resolved Analysis of N-RNA Interactions during RVFV Infection Shows Qualitative and Quantitative Shifts in RNA Encapsidation and PackagingVirusesDecember 2, 2021Miyuki Hayashi, Eric P. Schultz, Jean-Marc Lanchy, J. Stephen Lodmellovernight. Antibodies used for the blotting were anti-FLAG (Sigma, F3165, 1:2000), anti-GAPDH (BioLegend, 607901, 1:1000), anti-histone H2B (BioLegend, 606301, 1:1000), anti-mouse (Sigma, A2554, 1:6000), and anti-rat

(9) Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagePLOS PathogensNovember 15, 2021Zhe Zhao et al.anti-Myc (sc-40) and rabbit anti-paxillin (sc-5574) were from Santa Cruz Biotechnology. Mouse anti-GAPDH (607902) was from Biolegend). Mouse anti-pY31 paxillin was from BD Biosciences. Rabbit anti phospho-p44/42 MAPK

(10) An additional NF-κB site allows HIV-1 subtype C to evade restriction by nuclear PYHIN proteinsCell ReportsSeptember 21, 2021Matteo Bosso et al.(Santa Cruz Biotechnology, sc-8023), mouse anti-GAPDH (Santa Cruz Biotechnology, sc-365062), rat anti-GAPDH (BioLegend, 607902), rabbit anti-NFκB p65 (Santa Cruz Biotechnology, sc-372), mouse anti-HA (Abcam, ab18181), rabbit anti-Sp1

(11) APOBEC3F Constitutes a Barrier to Successful Cross-Species Transmission of Simian Immunodeficiency Virus SIVsmm to HumansJournal of virologyAugust 10, 2021Nchioua R, Kmiec D, Gaba A, Stürzel CM, Follack T, Patrick S, Kirmaier A, Johnson WE, Hahn BH, Chelico L, et al.

(12) IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitroNature communicationsJuly 28, 2021Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Krüger J, Schütz D, Heller S, Stürzel CM, Kmiec D, Conzelmann C, et al.(Cell Signaling #13202; 1:1000), mouse anti-FLAG (Sigma #F1804; 1:000), rat anti-GAPDH (Biolegend #607902, 1:1000) and SARS CoV-2N (anti-SARS-CoV-2N Sino Biologicals #40588-V08B, 1:1000).and Infrared Dye labeled

(13) Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilitiesCell reportsMay 18, 2021Hayn M, Hirschenberger M, Koepke L, Nchioua R, Straub JH, Klute S, Hunszinger V, Zech F, Prelli Bozzo C, Aftab W, et al.,Cell Signaling Technology,Cat#13202S; RRID:AB_2687461 "Monoclonal rat anti-GAPDH antibody, W17079A (1:1,000, wb)",Biolegend,Cat#607902; RRID:AB_2734503 "Polyclonal guinea pig anti-p62 antibody, (1:1,000, wb)",ProGen,Cat#GP62-N; RRID:AB_2801426 "Polyclonal

(14) IFI16 knockdown in primary HIV-1 target cellsSTAR protocolsMarch 19, 2021Bosso M, Bozzo CP, Volcic M, Kirchhoff F.

(15) Inhibition of Ganglioside Synthesis Suppressed Liver Cancer Cell Proliferation through Targeting Kinetochore Metaphase SignalingMetabolitesMarch 15, 2021Ting Su, Xian-Yang Qin, Naoshi Dohmae, Feifei Wei, Yutaka Furutani, Soichi Kojima, Wenkui Yuanti-AURKB (1:1000 dilution; 3094, Cell Signaling Technology), rat anti-human GAPDH (1:1000 dilution; 607902, BioLegend), and rabbit anti-human Lamin B1 (1:1000 dilution; ab16048, Abcam, Cambridge, MA, USA) antibodies

(16) Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 InfectionCellJanuary 7, 2021Wei J, Alfajaro MM, DeWeirdt PC, Hanna RE, Lu-Culligan WJ, Cai WL, Strine MS, Zhang SM, Graziano VR, Schmitz CO, et al.AB_315946 Anti-LMNB1 antibody,BioLegend,Cat#869802; RRID: AB_2820181 Anti-GAPDH antibody,BioLegend,Cat#607902; RRID: AB_2734503 Anti-HMGB1 antibody,Abcam,Cat#ab18256; RRID: AB_444360 Anti-SMARCA4 antibody,Abcam,Cat#ab70558;

(17) IL-10 and class 1 histone deacetylases act synergistically and independently on the secretion of proinflammatory mediators in alveolar macrophagesPloS oneJanuary 1, 2021Stanfield BA, Purves T, Palmer S, Sullenger B, Welty-Wolf K, Haines K, Agarwal S, Kasotakis G.mouse anti-phospho-IκBα S32/36 (Cell Signaling Technology, 9246S), and mouse anti-GAPDH (Biolegend, 607902) was then diluted to 1 μg/mL in 5% BSA-TBS-T and incubated at 4˚C overnight with agitation. The membrane

(18) HIV-1 infection activates endogenous retroviral promoters regulating antiviral gene expressionNucleic acids researchNovember 4, 2020Srinivasachar Badarinarayan S, Shcherbakova I, Langer S, Koepke L, Preising A, Hotter D, Kirchhoff F, Sparrer KMJ, Schotta G, Sauter D.#sc-160353), HA-Tag (abcam, ab 18181), V5-Tag (Cell Signaling Technology, #13202), GAPDH (BioLegend, #607902) and Infrared Dye labeled secondary antibodies (LI-COR IRDye, #926-68070, #926-68076, #926-68074, #926-32210,

(19) SARS-CoV-2 Is Restricted by Zinc Finger Antiviral Protein despite Preadaptation to the Low-CpG Environment in HumansmBioOctober 16, 2020Nchioua R, Kmiec D, Müller JA, Conzelmann C, Groß R, Swanson CM, Neil SJD, Stenger S, Sauter D, Münch J, et al.50, or anti-TRIM25 (BD Biosciences, catalog no. 610570) or with anti-GAPDH (BioLegend, catalog no. 607902) or anti-beta-actin (abcam, catalog no. ab8227), each diluted 1 to 2,000, or anti-ISG15 (Santa Cruz Biotechnology

(20) Nuclear PYHIN proteins target the host transcription factor Sp1 thereby restricting HIV-1 in human macrophages and CD4+ T cellsPLoS pathogensAugust 1, 2020Bosso M, Prelli Bozzo C, Hotter D, Volcic M, Stürzel CM, Rammelt A, Ni Y, Urban S, Becker M, Schelhaas M, et al.MNDA (Santa Cruz #sc-390739), AIM2 (Santa Cruz #sc-515514), GFP/BFP (Abcam #ab290) GAPDH (BioLegend #607902 or Santa Cruz #sc-365062), Sp1 (Abcam #157123 or Abcam #227383) and Infrared Dye labeled secondary antibodies

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Purified anti-GAPDH from BioLegend

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Purified anti-GAPDH from BioLegend

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