Fig 1: Induction and robust development of PDPN+ reticular networks in TA-TLS are regulated by TNFR and LTbR signaling, respectivelyDay 14 I.P. B16-OVA tumors from WT mice, WT mice treated with LTßR-Ig fusion protein, TNFR1/2-/- mice, Rag1-/- mice, and Rag1-/- mice repleted with CD8 T cells and/or B cells 3 days prior to tumor implantation were analyzed by IF or flow cytometry as described in STAR Methods. (A, E, and F) Scale bars: 100 µm. Data are from one experiment; n = 3–5 tumors per group.(B, C, G, and H) Data are from one experiment; n = 5 tumors per group.(D and I) CAF from day 14 i.p. tumors from WT mice, WT mice treated with LTßR-Ig, or TNFR1/2-/- mice were purified and expression of the indicated RNA transcripts determined as described in STAR Methods. Data are from two experiments presented as 2-?CT relative to Hprt; n = 6 tumors per group.Results are mean ± SD analyzed using unpaired Welch’s t test (A–D and F–I) or Kruskal-Wallis H test with Dunn’s post-test (E). nsp > 0.05, *p < 0.05, **p < 0.01.