Fig 1: Expression of synaptic vesicle proteins SV2A/B/C and SYT1/2 in MNs generated according to the protocols by Du et al. [35], Kroehne et al. [37] and Maury et al. [36] as well as SiMa cells were detected via Western blot using isoform-specific antibodies for detection. Each panel depicts the detection with one of the antibodies. A total of 10 µg of lysate pooled from three independent differentiations was analyzed and compared with mouse brain synaptosomes (mSYSOs). For SV2A and SV2B blots, the signal intensity from mSYSOs was far greater than for the other samples, and images with different exposure times were combined (Exposure time of cell lysates: SV2A 1 min, SV2B 10 min and for mSYSOs 2 s and 10 s, respectively).