ACSA-2 Antibody, anti-mouse, APC 130-117-386 from Miltenyi Biotec

Product Specs
ItemACSA-2 Antibody, anti-mouse, APC
CompanyMiltenyi Biotec
Price Pricing Info Supplier Page View Company Product Page
Catalog Number130-117-386
Quantity150 µg in 1 mL
ApplicationsFlow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
Conjugate/TagAPC
Reactivitymouse
IsotypeIgG2b
CloneIH3-18A3
Hostrat
Antibody TypePrimary antibodies
Clonalitymonoclonal
Concentration1:50
FormatReagents are supplied in buffer containing stabilizer and 0.05% sodium azide
TargetACSA-2
Research AreaFor research use only
Regulatory StatusFor research use only

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Miltenyi Biotec

Miltenyi Biotec Inc.

2303 Lindbergh Street

Auburn, CA 95602

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Phone: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925

Email: macs@miltenyibiotec.com

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Website: www.miltenyibiotec.com

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Miltenyi Biotec

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone: +49 2204 8306-0
Fax: +49 2204 8306-235

Email: macs@miltenyibiotec.de

Company Profile

Website: www.miltenyibiotec.com

(1) CellNeighborEX: deciphering neighbor-dependent gene expression from spatial transcriptomics dataMolecular systems biologyNovember 9, 2023Kim H, Kumar A, Lövkvist C, Palma AM, Martin P, Kim J, Bhoopathi P, Trevino J, Fisher P, Madan E, et al.the hippocampus cells were suspended in FACS buffer 2 and stained with ACSA2: APC (Miltenyi Biotec #130‐117‐535) and CD11b: BUV395 (BD Biosciences #565976). Here, the SSC/CD11b gate was used to select CD11b negative

(2) Accelerated aging in mice with astrocytic redox imbalance as a consequence of SOD2 deletionAging CellSeptember 1, 2023Konstantinos Tsesmelis, Gandhari Maity‐Kumar, Dana Croner, Jasmin Sprissler, Miltiadis Tsesmelis, Tabea Hein, Bernd Baumann, Thomas Wirththe isolated fractions by flow cytometry against astrocytic‐specific marker ACSA‐2 (Miltenyi Biotec, #130‐117‐535). The flow cytometry was performed in BD FACS CANTO II flow cytometer (BD Biosciences). The software

(3) Brain cell type specific proteomics approach to discover pathological mechanisms in the childhood CNS disorder mucolipidosis type IVFrontiers in Molecular NeuroscienceAugust 7, 2023Madison Sangster, Sanjid Shahriar, Zachary Niziolek, Maria Carla Carisi, Michael Lewandowski, Bogdan Budnik, Yulia Grishchukcell labelling. The cells were then labelled with the following antibodies: anti-ASCA-2-APC (Miltenyi #130–117-535, RRID:AB_2727978; 1:14) for astrocytes, anti-O4-488 (R&D #FAB1326G, RRID:AB_2936942; 1:27) for oligodendrocytes,

(4) Microglia-derived TGF-β1 ligand maintains microglia homeostasis via autocrine mechanism and is critical for normal cognitive function in adult mouse brainbioRxivJuly 12, 2023Alicia Bedolla, Elliot Wegman, Max Weed, Aditi Paranjpe, Anastasia Alkhimovitch, Igal Ifergan, Lucas McClain, Yu Luoprotein expressed by Cx3cr1CreER-R26-YFP for microglia collection, and ASCA-2 APC conjugated antibody (130-117-535, Miltenyi Biotec) for astrocytes. Any double positive cells were excluded from the gating to improve

(5) Haslea ostrearia Pigment Marennine Affects Key Actors of Neuroinflammation and Decreases Cell Migration in Murine Neuroglial Cell ModelInternational Journal of Molecular SciencesMarch 11, 2023Sarah Méresse, Hélène Gateau, Tessa Tirnan, Vanessa Larrigaldie, Nathalie Casse, Pamela Pasetto, Jean-Luc Mouget, Stéphane Mortaud, Mostefa Fodilantibodies: Fixable Viability Dye 65-0865-14, 1/800 (eBiosciences™, San Diego, CA, USA), anti-ACSA2 APC 130-117-386, 1:50 (MILTENYI Biotec, Bergisch Gladbach, ), anti-CD45 V450 560501, 1:100 (BD Horizon™, San Jose, CA,

(6) GSK3β Inhibition by Phosphorylation at Ser389 Controls NeuroinflammationInternational Journal of Molecular SciencesDecember 25, 2022Belen Calvo, Miriam Fernandez, Mercedes Rincon, Pedro TranqueTargets,Concentration,Company(Reference) ACSA-2-APC,IH3-18A3,Neonatal and adultAstrocytes,1:25,Miltenyi Biotec(130-117-535) B220 (CD45R),RA3-6B2,B lymphocytes,1:25,Miltenyi Biotec (130-102-259) Calibration beads,,,1000 beads/mL,Miltenyi Biotec

(7) Astrocyte Cell Surface Antigen 2 and Other Potential Cell Surface Markers of Enteric glia in the Mouse ColonASN NeuroMay 20, 2022Vladimir Grubišić, Brian D. GulbransenNo.,RRID,Dilution Primary Antibodies,,, APC-conjugated anti-mouse ACSA2 [IH3-18A3],Miltenyi Biotec Cat# 130-117-535,AB_2727978,1:50 APC-conjugated anti-mouse ACSA2 [REA969],Miltenyi Biotec Cat# 130-116-245,AB_2727423,1:50 Biotinylated

(8) Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunctionMolecular therapy. Nucleic acidsDecember 3, 2021Pan J, Ma N, Zhong J, Yu B, Wan J, Zhang W.PE-Cy7-labeled CD45 (103114, BioLegend), PE-labeled CD11b (101208, BioLegend), and APC-labeled ACSA2 (130-117-386, Miltenyi Biotec) antibodies. The cells were then rinsed in PBS, centrifuged, resuspended in FACS buffer

(9) Rescue of a lysosomal storage disorder caused by Grn loss of function with a brain penetrant progranulin biologicCellSeptember 2, 2021Logan T, Simon MJ, Rana A, Cherf GM, Srivastava A, Davis SS, Low RLY, Chiu CL, Fang M, Huang F, et al.#564406, 1:100) to exclude dead cells, CD11b-BV421 (BD Biosciences 562605, 1:100), ACSA2-APC (Miltenyi #130–117-386, 1:100), and Thy1-PE (R&D #FAB7335P, 1:100). Cells were washed with PBS/1% BSA and strained through

(10) Analysis of glucose metabolism by ¹⁸F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhageScientific reportsMay 25, 2021Han X, Ren H, Nandi A, Fan X, Koehler RC.the primary antibodies: CD11b-FITC (Miltenyi Biotec, 130–113-234) and ACSA-2-APC (Miltenyi Biotec, 130–117-535) or and Ly6G-APC (Pharmingen, 560,599) for 30 min at 4ºC. The corresponding isotype antibodies were

(11) Dissociation of neonatal and adult mice brain for simultaneous analysis of microglia, astrocytes and infiltrating lymphocytes by flow cytometryIBRO reportsJune 1, 2020Calvo B, Rubio F, Fernández M, Tranque P.radial glia,1:25,Miltenyi Biotec (130-118-483) ACSA-2-APC,IH3-18A3,Neonatal and adult astrocytes,1:25,Miltenyi Biotec (130-117-535) FcR Blocking Reagent,,,1:100,Miltenyi Biotec (130-092-575) Calibration Beads,,,1000 beads/ml,Miltenyi Biotec

(12) Transcriptomic profiling of microglia and astrocytes throughout agingJournal of neuroinflammationApril 1, 2020Pan J, Ma N, Yu B, Zhang W, Wan J.PE-Cy7-labeled CD45 (103114, BioLegend), PE-labeled CD11b (101208, BioLegend), and APC-labeled ACSA2 (130-117-386, Miltenyi Biotec). The cells were then rinsed in PBS, centrifuged, resuspended in FACS buffer (1% FBS

(13) An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibodyThe Journal of biological chemistryMay 26, 2017Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG.

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ACSA-2 Antibody, anti-mouse, APC from Miltenyi Biotec

Miltenyi Biotec

Miltenyi Biotec

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ACSA-2 Antibody, anti-mouse, APC from Miltenyi Biotec

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