Fig 1: Frequency of immune-cell subsets between HDs (n = 18) and patients with COVID-19 (n = 50)(A) Frequency of total CD3 T cells, CD4 and CD8 T cell subsets, and activated CD38+HLADR+ CD8 T cells.(B) Frequency of B cell subsets (CD21+CD27+: resting memory, CD21−CD27+: activated memory, CD21−CD27-: exhausted) and plasmablasts (CD38++CD27+) gated on CD19+ B cells.(C) Frequency of NK cell subsets (gated on CD3- CD14-) CD56Bright: CD56++CD16+, CD56dim: CD56+CD16++CD57+/−, differentiated Ki67+ NK cells (gated on CD56Bright or CD56dimCD57- NK cells) and differentiated Ki67+ NKT cells (gated on CD3+CD56+ cells).(D) Monocyte subsets (gated on CD3−CD56-) (classical monocytes: CD14+CD16-, intermediate monocytes: CD16+CD14+, non-classical monocytes: CD14−CD16+).(E) Frequency of γδ T cells (gated on CD3+ T cells) and CD16 and NKG2A expression (gated on γδ CD3 T cells).(F) Frequency of DC subsets (gated on HLADR+Lin−) (pDC: CD45RA+CD33−CD123+, pre-DC: CD123+CD45RA+, cDC1: CD33+CD123−CD141+CD1clow, cDC2: CD33+CD123−CD14+CD1c+) detected by flow cytometry in PBMCs from n = 50 patients with COVID-19 and n = 18 HDs. The differences between the two groups were evaluated using Wilcoxon rank sum statistical tests. The lower and upper boundaries of the box represent the 25% and 75% percentiles, the whiskers extend to the most extreme data point that is no more than 1.5 times the interquartile range away from the box. Median values (horizontal line in the boxplot) are shown.See also Figures S1 and S2.