Fig 2: Protein expression of tyrosine hydroxylase (TH) and phenylethanolamine N-methyltransferase (PNMT) in MCF-10A or in MCF-7 cells, revealed by immunocytochemistry. Shown are representative microphotographs of immunoreactivities revealed by secondary antibodies conjugated with Alexa fluor 594 (red fluorescence, TH) or with Alexa fluor 488 (green fluorescence, PNMT). Nuclei were labelled with Hoechst 33342 (blue fluorescence). Scale bar: 20 µm. Negative control (CTR): without primary antibody.