Fig 2: Intracellular CD3 marks thymus-dependent ILC precursors in peripheral tissues(A) Analyses of lung ILCs in indicated strains. The lineage cocktail and special efforts to exclude T and NK cells are described in the STAR Methods. ST2+ and ST2− subsets are color coded in blue and red. Bar graphs show cumulative data from at least three experiments. Unpaired Student's test was used for determining the statistical significance. ∗p<0.05, ∗∗<0.01, ∗∗∗p<0.001, ∗∗∗∗p<0.0001.(B) Analyses of small intestine ILCs in indicated strains. KLRG1+ and KLRG1− subsets are color coded in red and green. Bar graphs show cumulative data from at least three experiments. One-way ANOVA was used for determining the statistical significance. ∗∗<0.01, ∗∗∗p<0.001, ∗∗∗∗p<0.0001. Data are represented as mean ± SD.

Fig 3: icCD3e+ cells mature into ILC2 and ILC3 in the lung(A) Lung Lin-CD127+ cells of 2-month-old WT mice were analyzed for the expression of indicated transcription factors in triplicates. Subsequent gates from ST2- and ST2+ cells are color coded in red and blue. Mean fluorescence intensities of icCD3e and GATA3 in indicated subsets are plotted on the right.(B) Analyses of Lin-CD127+ cells of WT lung at indicated time points post subcutaneous infection with 500 stage3 Nb. Cell numbers or MFI of icCD3e of indicated subsets are shown in the bar graphs.(C) Cytokine expression in the indicated subsets from experiments described in (B) was measured after stimulation with PMA and Ionomycin for 3.5 h. One-way ANOVA was used for determining the statistical significance. *p<0.05, **<0.01, ***p<0.001, ****p<0.0001. Data are represented as mean ± SD.