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Antibodies
Anti-CRISPR-Cas9 antibody [7A9-3A3]
Anti-CRISPR-Cas9 antibody [7A9-3A3] from Abcam
Be the first to write a review!
Citations:
(57)
Supplier Page
Supplier Page from
Abcam for
Anti-CRISPR-Cas9 antibody [7A9-3A3]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-CRISPR-Cas9 antibody [7A9-3A3]
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab191468
Quantity
10 UG, 100 UG
Applications
Immunocytochemistry/ Immunofluorescence, Western blot, Immunocytochemistry/ Immunofluorescence, Western blot
Conjugate/Tag
Unconjugated
Host
Mouse, Nude mouse
Antibody Type
Primary
Clonality
Monoclonal
Clone
7A9-3A3
Gene Name
cas9
Reactivity
Streptococcus pyogenes
Target
CRISPR-Cas9
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (57)
(1)
Inhibition of lysosome-tethered Ragulator-Rag-3D complex restricts the replication of Enterovirus 71 and Coxsackie A16
Journal of Cell Biology
December 4, 2023
Xinhui Wang, Zhilin Hu, Wei Zhang, Shuwei Wu, Yongjin Hao, Xia Xiao, Jingjing Li, Xiaoliang Yu, Chengkui Yang, Jingfeng Wang,
et al.
Enterovirus
71 3D (Cat #
GTX630193
, RRID:AB_2888196;
GeneTex
),
GSDME
(Cat #
ab215191
;
Abcam
), Cas9 (Cat #
ab191468
;
Abcam
),
Caspase-3
(Cat #
9662s
;
Cell Signaling Technology
), Cleaved-
Caspase-3
(Cat #
9661
;
Cell Signaling Technology
(2)
Lipid Nanoparticle-Mediated Hit-and-Run Approaches Yield Efficient and Safe In Situ Gene Editing in Human Skin
ACS Nano
November 14, 2023
Juliana Bolsoni, Danny Liu, Fatemeh Mohabatpour, Ronja Ebner, Gaurav Sadhnani, Belal Tafech, Jerry Leung, Selina Shanta, Kevin An, Tessa Morin,
et al.
purchased from Fisher Scientific (Mississauga, ON, Canada). Mouse monoclonal antibody against Cas9 (
ab191468
) and live and dead cell
assays
were purchased from
Abcam
(Cambridge, MA, USA). ApoE4 (Apolipoprotein
(3)
CRISPR/dCAS9-mediated DNA demethylation screen identifies functional epigenetic determinants of colorectal cancer
Clinical Epigenetics
August 24, 2023
Juan Ramón Tejedor, Alfonso Peñarroya, Javier Gancedo-Verdejo, Pablo Santamarina-Ojeda, Raúl F. Pérez, Sara López-Tamargo, Ana Díez-Borge, Juan J. Alba-Linares, Nerea González-del-Rey, Rocío G. Urdinguio,
et al.
using the following primary antibodies (
1:1000
dilution in TBS-T with 5% milk): anti-Cas9 (
Abcam
, #
ab191468
) and anti- α Tubulin (
Abcam
, #
ab7291
). The secondary antibody was a
rabbit anti-mouse IgG H&L
(
Abcam
,
(4)
Lipopolysaccharide transport regulates bacterial sensitivity to a cell wall-degrading intermicrobial toxin
PLOS Pathogens
June 26, 2023
Kristine L. Trotta, Beth M. Hayes, Johannes P. Schneider, Jing Wang, Horia Todor, Patrick Rockefeller Grimes, Ziyi Zhao, William L. Hatleberg, Melanie R. Silvis, Rachel Kim,
et al.
hour at RT with shaking in 3% milk+TBST. Primary antibody was applied:
1:1000
mouse
anti-Cas9 (
Abcam
ab191468
) in TBST, overnight, at 4C with shaking. Blots were washed four times in TBST. Secondary antibody was
(5)
Rapid and Reliable Quantification of Prime Editing Targeting Within the Porcine ABCA4 Gene Using a Bioluminescence Resonance Energy Transfer-Based Sensor
Nucleic Acid Therapeutics
June 1, 2023
Tobias Wimmer, Hannah Sawinski, Anne M. Urban, Jan Motlik, Knut Stieger
of prime editors in HEK293-
T cells
with an antibody raised against SpCas9 (
mouse
anti-CRISPR-Cas9
;
ab191468
;
Abcam
, Cambridge, United Kingdom). HEK293-
T cells
transfected
with PEs were lysed as described above.
(6)
Lipopolysaccharide integrity primes bacterial sensitivity to a cell wall-degrading intermicrobial toxin
bioRxiv
May 6, 2023
Kristine L Trotta, Beth M Hayes, Johannes P Schneider, Jing Wang, Horia Todor, Patrick Rockefeller Rockefeller Grimes, Ziyi Zhao, William L Hatleberg, Melanie R Silvis, Rachel Kim,
et al.
hour at RT with shaking in 3% milk+TBST. Primary antibody was applied:
1:1000
mouse
anti-Cas9 (
Abcam
ab191468
) in TBST, overnight, at 4C with shaking. Blots were washed four times in TBST. Secondary antibody was
(7)
Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma
Clinical epigenetics
April 29, 2023
Sgro A, Cursons J, Waryah C, Woodward EA, Foroutan M, Lyu R, Yeoh GCT, Leedman PJ, Blancafort P.
polyclonal (
Abcam
, Cat. No.
ab122507
,
1:300
), and α-SpCas9
mouse
monoclonal [7A9-3A3] (
Abcam
, Cat. No.
ab191468
,
1:500
). The secondary antibodies used were Alexa Fluor 488-conjugated
goat
α-
mouse
(Thermo Fisher Scientific,
(8)
A molecular glue approach to control the half-life of CRISPR-based technologies
bioRxiv
March 24, 2023
Vedagopuram Sreekanth, Max Jan, Kevin T. Zhao, Donghyun Lim, Jessie R. Davis, Marie McConkey, Veronica Kovalcik, Sam Barkal, Benjamin K. Law, James Fife,
et al.
were subjected to
immunoblotting
with a monoclonal antibody anti-Cas9 antibody (
Abcam
:
ab189380
or
ab191468
), anti-FLAG antibody (
Cell Signaling Technologies
:
2368S
), and a monoclonal anti-β-actin antibody (
Cell Signaling Technologies
(9)
Genome-scale CRISPR screening in a single mouse liver
Cell Genom
December 14, 2022
Keys HR, Knouse KA.
(ThermoFisher Scientific). The following primary antibodies were used: Cas9 (
1:200
,
clone
7A9-3A3,
Abcam
ab191468
), asialoglycoprotein receptor 1 (ASGR1) (
1:500
,
clone
114,
Sino Biological
50083-R114
), mCherry (
1:500
,
(10)
Non-viral CRISPR activation system targeting VEGF-A and TGF-β1 for enhanced osteogenesis of pre-osteoblasts implanted with dual-crosslinked hydrogel
Materials Today Bio
December 1, 2022
Guo Chen, Shaohui Deng, Mingxiang Zuo, Jin Wang, Du Cheng, Bin Chen
0.1% Tween 20) for 1 h. Cells were incubated with a 200 × dilution of
anti-CRISPR-Cas9
antibody (
ab191468
,
Abcam
, Cambridge, UK) in PBST plus 1% BSA in a humidified chamber overnight at 4 °C. Cells were washed
(11)
A loss-of-adhesion CRISPR-Cas9 screening platform to identify cell adhesion-regulatory proteins and signaling pathways
Nature Communications
December 1, 2022
Martin F. M. de Rooij, Yvonne J. Thus, Nathalie Swier, Roderick L. Beijersbergen, Steven T. Pals, Marcel Spaargaren
lenti-Guide-Puro Brunello kinome library (Addgene #1000000082). Antibodies:
mouse
anti-Cas9 (
Clone
7A9-3A3, #
ab191468
,
Abcam
), phycoerythrin-conjungated
goat
F(ab’)2 anti-mouse IgG1 (Polyclonal, #
1072-09
,
Southern Biotech
),
(12)
Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing
Nature Communications
December 1, 2022
Duško Lainšček, Vida Forstnerič, Veronika Mikolič, Špela Malenšek, Peter Pečan, Mojca Benčina, Matjaž Sever, Helena Podgornik, Roman Jerala
antibodies. Primary antibodies used
for western blotting
were as follows: mouse anti-CRISPR-Cas9 antibody (
ab191468
) from
Abcam
and rabbit
anti-Alpha/Beta tubulin
antibody (
2148
) that were purchased from
Cell Signaling Technology
(13)
Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages
Cell Reports
September 6, 2022
Hironori Hojo et al.
RRID:AB_731605 Rabbit polyclonal anti-CBFb antibody,
Abcam
,
ab195411
Mouse
monoclonal
anti-CRISPR-Cas9
antibody,
Abcam
,
ab191468
; RRID:AB_2692325 Rabbit polyclonal to Histone H3 antibody,
Abcam
,
ab1791
; RRID:AB_302613 Alexa Fluor
(14)
CAPTURE of the Human U2 snRNA Genes Expands the Repertoire of Associated Factors
Biomolecules
May 14, 2022
Guiro J, Fagbemi M, Tellier M, Zaborowska J, Barker S, Fournier M, Murphy S.
Antibodies,Reference AffiniPure
Donkey Anti-Rabbit IgG (H + L
),
Jackson ImmunoResearch
711-005-152
Anti-CRISPR-Cas9
,
Abcam
ab191468
CDK12
,
LS Bio
LS-C288466
-100
CDK12
,
Novus Biologicals
NB100-87011
Cyclin K,
Bethyl Laboratories
A301-939A
Oct-1
,
Bethyl Laboratories
(15)
Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
Nature Communications
December 1, 2021
Daniel M. Sapozhnikov, Moshe Szyf
TBST and protein was detected with either
mouse
Anti-CRISPR-Cas9
primary antibody [7A9-3A3] (
Abcam
,
ab191468
) (1/2000 dilution) or monoclonal Anti-β-Actin primary antibody produced in
mouse
(
Sigma
,
A2228
) (1/5000
(16)
Broadening the reach and investigating the potential of prime editors through fully viral gene-deleted adenoviral vector delivery
Nucleic Acids Research
November 18, 2021
Qian Wang, Jin Liu, Josephine M Janssen, Francesca Tasca, Hailiang Mei, Manuel A F V Gonçalves
incubated overnight at 4°C with primary antibodies raised against S. pyogenes Cas9 (
Abcam
; Cat. No.:
ab191468
;
1:1000
dilution), myosin heavy chain (
Sigma-Aldrich
; Cat. No.:
M4276
;
1:500
dilution), sarcomeric α-actinin
(17)
Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex
Science advances
October 8, 2021
Ban Y, Yu T, Feng B, Lorenz C, Wang X, Baker C, Zou Y.
anti-
synaptic vesicle
2 (SV2) (Developmental Studies Hybridoma Bank), and
anti–CRISPR-Cas9
(
Abcam
,
ab191468
). Hippocampal
neuron
and HEK293T
coculture
The mixed culture
assay
was performed as described with
(18)
Activation of HERV-K(HML-2) disrupts cortical patterning and neuronal differentiation by increasing NTRK3
Cell stem cell
September 2, 2021
Padmanabhan Nair V, Liu H, Ciceri G, Jungverdorben J, Frishman G, Tchieu J, Cederquist GY, Rothenaigner I, Schorpp K, Klepper L,
et al
.
or RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies
Mouse
monoclonal Anti-CAS9,
Abcam
,"
ab191468
, RRID:AB_2692325" Mouse monoclonal Anti-GAPDH,
EMD Millipore
Corp,"
CB1001–500UG
, RRID:AB_2107426"
Mouse
(19)
Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic Kras(G12D) in pancreatic cancer
Life science alliance
September 1, 2021
McAndrews KM, Xiao F, Chronopoulos A, LeBleu VS, Kugeratski FG, Kalluri R.
9102
" Phospho-
p44
/42 MAPK (Erk1/2) (Thr202/Tyr204),Rabbit,"
1:1,000
","
CST
,
4376
" Cas9,
Mouse
,
1:500
,"
Abcam
,
ab191468
" HRP-conjugated β-actin,-,"
1:25,000
","
Sigma-Aldrich
,
A3854
" Anti-rabbit HRP-conjugated,-,"
1:5,000
","
CST
,
(20)
Control of gasdermin D oligomerization and pyroptosis by the Ragulator-Rag-mTORC1 pathway
Cell
August 19, 2021
Evavold CL, Hafner-Bratkovič I, Devant P, D'Andrea JM, Ngwa EM, Boršić E, Doench JG, LaFleur MW, Sharpe AH, Thiagarajah JR,
et al
.
from Takara (631131,
1:1000
), spCas9 from
Cell Signaling Technologies
(
65832S
,
1:1000
) and from
Abcam
(
ab191468
,
1:1000
),
RagA
from
Cell Signaling Technologies
(
4357S
,
1:1000
), RagC from
Cell Signaling Technologies
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Anti-CRISPR-Cas9 antibody [7A9-3A3] from Abcam
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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