Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker ab51317 from Abcam

Product Specs
ItemAnti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab51317
Quantity10 UG, 100 UG
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
HostRat
Antibody TypePrimary
ClonalityMonoclonal
CloneE13 161-7
Gene NameLy6a
ReactivityMouse
TargetSca1 / Ly6A/E

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Mechanically induced pyroptosis enhances cardiosphere oxidative stress resistance and metabolism for myocardial infarction therapyNature CommunicationsOctober 2, 2023Yingwei Wang, Qi Li, Jupeng Zhao, Jiamin Chen, Dongxue Wu, Youling Zheng, Jiaxin Wu, Jie Liu, Jianlong Lu, Jianhua Zhang, et al.(1:200, ab81289, Abcam), CD90 (1:200, ab225, Abcam), CD105 (1:200, ab156756, Abcam), Sca-1 (1:200, ab51317, Abcam), and KDR (1:200, sc6251, Santa Cruz) were determined by flow cytometry. After a 3-day cultivation,

(2) Dual stimulus responsive borosilicate glass (BSG) scaffolds promote diabetic alveolar bone defectsrepair by modulating macrophage phenotypeBioactive MaterialsAugust 1, 2023Pengfei Tian, Limin Zhao, Jua Kim, Xian Li, Chunyu Liu, Xu Cui, Tao Liang, Yunbo Du, Xiehui Chen, Haobo PanARG(PA5-29645), PINK1 (PA1-16604), PARKIN (PA5-13399) were purchased from Thermo Fisher. CD90 (ab181469), sca1 (ab51317) were purchased from Abcam. ELISA kits for mouse TGF-β were obtained from Beyotime. Percent-positive

(3) Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectorsScience AdvancesApril 21, 2023Timothy F. Shay, Erin E. Sullivan, Xiaozhe Ding, Xinhong Chen, Sripriya Ravindra Kumar, David Goertsen, David Brown, Anaya Crosby, Jost Vielmetter, Máté Borsos, et al.performed on HEK293T cells to label transiently transfected receptors such as LY6A (1:200 dilution; Abcam, ab51317), LY6C1 (1:200 dilution; Abcam, ab15627), and CA-IV (1:40 dilution; Invitrogen, PA5-47312). HEK293T

(4) Deciphering postnatal limb development at single-cell resolutioniScienceJanuary 20, 2023Manman Gao, Xizhe Liu, Peng Guo, Jianmin Wang, Junhong Li, Wentao Wang, Martin J. Stoddart, Sibylle Grad, Zhen Li, Huachuan Wu, et al.RRID: AB_2785500 Ly6e antibody,Biorbyt,Cat# orb20596; RRID: AB_10751664 Anti-Sca1 antibody,Abcam,Cat# ab51317; RRID: AB_1640946 Tnni2 antibody,ABclonal,Cat# A4740; RRID: AB_2863337 Osteopontin antibody,Affinity Biosciences

(5) Multilineage commitment of Sca-1(+) cells in reshaping vein graftsTheranosticsJanuary 1, 2023Ni Z, Lyu L, Gong H, Du L, Wen Z, Jiang H, Yang H, Hu Y, Zhang B, Xu Q, et al.counted in each section as indicated. Primary antibodies used in this study included Sca-1 (Abcam, ab51317, 1:200), tdTomato (Rockland, 600-401-379, 1:500), α-SMA (Sigma, A5228, 1:500), SM22 (Abcam, ab14106,

(6) Dexmedetomidine alleviates inflammatory response and oxidative stress injury of vascular smooth muscle cell via α2AR/GSK-3β/MKP-1/NRF2 axis in intracranial aneurysmBMC Pharmacology and ToxicologyOctober 23, 2022Ze Zhang, Xiue Mu, Xiaohui Zhou1:50; Abcam) and stem cell markers CD44 (ab238464, 0.474 μg/ml; Abcam), stem cell antigen-1 (Sca1; ab51317, 0.2 μg/106 cells; Abcam) and S10 calcium-binding protein B (S100β; Thermo Fisher Scientific, Waltham,

(7) SENP1 regulates the transformation of lung resident mesenchymal stem cells and is associated with idiopathic pulmonary fibrosis progressionCell communication and signaling : CCSJuly 14, 2022Sun W, Liu X, Yang X, Jing X, Duan C, Yang G, Wu C, Huang H, Luo Q, Xia S, et al.antibodies against α-SMA (Abcam, Ab240654, 1:200), SENP1 (Abcam, Ab236094, 1:200), and Sca-1 (Abcam, Ab51317, 1:200) were incubated with the sections overnight at 4 °C. Secondary antibodies were incubated with

(8) X-box binding protein 1–mediated COL4A1s secretion regulates communication between vascular smooth muscle and stem/progenitor cellsJournal of Biological ChemistryMarch 15, 2021Angshumonik Angbohang et al.GenScript using peptide CRSSQRTQKDPVPY (XBP1u) and DSGGIDSSDSESDIC (XBP1s). The antibodies against Sca-1 (ab51317), IRE1α (ab37073), COL4A1 (ab6586), Col15A1 (ab58717), and Col18A1 (ab52022) were purchased from Abcam;

(9) GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activityTheranosticsJanuary 1, 2021Guo S, Gu J, Ma J, Xu R, Wu Q, Meng L, Liu H, Li L, Xu Y.and 1% penicillin/streptomycin. To characterize the OMSC markers, Sca-1 (stem cell antigen-1; Abcam, ab51317), CD73 (proteintech, 12231-1-AP) were incubated by immunocytochemistry. Primary bone marrow-derived macrophages

(10) Gli1⁺ Cells Couple with Type H Vessels and Are Required for Type H Vessel FormationStem cell reportsJuly 14, 2020Chen J, Li M, Liu AQ, Zheng CX, Bao LH, Chen K, Xu XL, Guan JT, Bai M, Zhou T, et al.1:100), NG2 (ab5320, Millipore, USA; diluted 1:100), CD146 (ab75769, Abcam, UK; diluted 1:100), SCA1 (ab51317, Abcam, UK; diluted 1:200), RUNX2 (12556, Cell Signaling Technology, USA; diluted 1:200), and HIF-1α

(11) A histone deacetylase 7-derived peptide promotes vascular regeneration via facilitating 14-3-3γ phosphorylationStem CellsApril 1, 2020Yang J, Moraga A, Xu J, Zhao Y, Luo P, Lao KH, Margariti A, Zhao Q, Ding W, Wang G, et al.antibodies in rabbit by GenScript (Piscataway, New Jersey). The antibodies against CD31 (ab28364), Sca‐1 (ab51317), 14‐3‐3γ (ab115176), and MEKK1 (ab55653) were purchased from Abcam (Cambridge, UK). The antibodies

(12) Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle RegenerationCell stem cellDecember 5, 2019Scott RW, Arostegui M, Schweitzer R, Rossi FMV, Underhill TM.ab11575, RRID:AB_298179" anti-CD31,BD,"Cat# 550274, RRID:AB_393571" anti-LY6A (Sca1),Abcam,"Cat# ab51317, RRID:AB_1640946" anti-CXCL5,Abcam,Cat# ab198505 anti-Perilipin,Cell Signaling,"Cat# 9349S, RRID:AB_10829911" anti-Periostin,Abcam,"Cat#

(13) Electrical Stimulation of pediatric cardiac-derived c-kit+ progenitor cells improves retention and cardiac function in right ventricular heart failureStem CellsDecember 1, 2019Joshua T. Maxwell et al.Biotechnology), and Nkx‐2.5 (14,033; Santa Cruz Biotechnology), Notch1 (ab8925, Abcam, Cambridge, UK), Sca‐1 (ab51317, Abcam), CD31 (sc‐28,188, Santa Cruz Biotechnology), or CD34 (7,324; Santa Cruz Biotechnology). Pooled

(14) Diabetes-Induced NF-κB Dysregulation in Skeletal Stem Cells Prevents Resolution of InflammationDiabetesNovember 1, 2019Ko KI, Syverson AL, Kralik RM, Choi J, DerGarabedian BP, Chen C, Graves DT.(ab34674), anti-F4/80 (ab6640), anti-CD4 (ab183685), anti-IKKβ (ab124957), anti-CD271 (ab52987), anti-Sca1 (ab51317), anti–inducible nitric oxide synthase (iNOS) (ab15323), anti-CD163 (ab182422), and anti–TGF-β1 (ab92486).

(15) Endothelial cell clonal expansion in the development of cerebral cavernous malformationsNature communicationsJune 24, 2019Malinverno M, Maderna C, Abu Taha A, Corada M, Orsenigo F, Valentino M, Pisati F, Fusco C, Graziano P, Giannotta M, et al.BD Biosciences); rabbit anti-Fn1 polyclonal (1:200; ab23750; AbCam); rat anti-Sca1 monoclonal (1:200; ab51317; AbCam); rabbit anti-Id1 polyclonal (1:200; sc-488; Santa Cruz); rabbit anti-Erg1/2/3 polyclonal (1:200;

(16) Long non‑coding RNA MALAT‑1 contributes to maintenance of stem cell‑like phenotypes in breast cancer cellsOncology LettersDecember 8, 2017Linghuan Zeng, Ying Cen, Junjie Chenoctamer-binding transcription factor 4 (Oct4; cat. no. ab19857) and stem cell antigen-1 (Sca-1; cat. no. ab51317) (Abcam, Cambridge, UK) were used in the present study. All antibodies were diluted in PBS at 1:2,000

(17) Stromal cyclin D1 promotes heterotypic immune signaling and breast cancer growthOncotargetOctober 10, 2017Pestell TG, Jiao X, Kumar M, Peck AR, Prisco M, Deng S, Li Z, Ertel A, Casimiro MC, Ju X, et al.

(18) Aberrant perichondrial BMP signaling mediates multiple osteochondromagenesis in miceJCI insightAugust 3, 2017Inubushi T, Nozawa S, Matsumoto K, Irie F, Yamaguchi Y.(catalog ab54236), rat polyclonal anti-CD44 (catalog ab25340), and rat monoclonal anti–Sca-1 (catalog ab51317) from Abcam; rabbit polyclonal anti-pSmad1/5/8 (catalog 9511) and rabbit monoclonal antiSox9 (catalog

(19) Aberrant Transforming Growth Factor-β Activation Recruits Mesenchymal Stem Cells During Prostatic HyperplasiaStem cells translational medicineFebruary 1, 2017Wang L, Xie L, Tintani F, Xie H, Li C, Cui Z, Wan M, Zu X, Qi L, Cao X.ab5694; Abcam, Cambridge, U.K., http://www.abcam.com), vimentin (1:200, ab92547; Abcam), Sca1 (1:100, ab51317; Abcam), and CD90 (1:100, ab3105; Abcam) overnight at 4°C. For immunohistochemical staining, biotinylated

(20) Heme Oxygenase-1/Carbon Monoxide System and Embryonic Stem Cell Differentiation and Maturation into CardiomyocytesAntioxidants & redox signalingMarch 1, 2016Suliman HB, Zobi F, Piantadosi CA.embedded, and sectioned. For immunofluorescence techniques, the sections were costained with Sca1 (Abcam; # Ab51317) and CS (Cell Signaling; #14309) or Nkx2.5 (Cell Signaling; #8792) and CS. The positive cells in ventricles

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Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker from Abcam

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Anti-Sca1 / Ly6A/E antibody [E13 161-7] - Hematopoietic Stem Cell Marker from Abcam

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