Anti-Desmin antibody - Cytoskeleton Marker ab15200 from Abcam

Product Specs
ItemAnti-Desmin antibody - Cytoskeleton Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab15200
Quantity1 ML, 100 UL
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
HostRabbit
Antibody TypePrimary
ClonalityPolyclonal
Gene Namedes
ReactivityHuman, Mouse, Rat
TargetDesmin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Betulin Accelerated the Functional Recovery of Injured Muscle in a Mouse Model of Muscle ContusionInternational Journal of Medical SciencesJanuary 1, 2024Yueh-Hsiu Lu, Yi-Fu Huang, Cheng-Pu Hsieh, Jr-Kai Chen, Hsuan-Ying Chen, Show-Mei Chuangembedded in paraffin and then hematoxylin and eosin, immunohistochemistry staining (anti-desmin, 1:100, ab15200, Abcam, Cambridge, MA) were performed for the histopathological muscle regeneration marker, and Masson's

(2) Lysosomal cholesterol overload in macrophages promotes liver fibrosis in a mouse model of NASHJournal of Experimental MedicineNovember 6, 2023Michiko Itoh, Atsushi Tamura, Sayaka Kanai, Miyako Tanaka, Yohei Kanamori, Ibuki Shirakawa, Ayaka Ito, Yasuyoshi Oka, Isao Hidaka, Taro Takami, et al.with Sirius red. Immunohistochemical staining was performed for F4/80 (MCA497GA; Serotec), desmin (ab15200; Abcam), CTSD (ab75852; Abcam), osteopontin (AF808; R&D), and type III collagen (1330-01; SouthernBiotech).

(3) Loss of Pla2r1 decreases cellular senescence and age‐related alterations caused by aging and Western dietsAging CellNovember 1, 2023Amélie Massemin, Delphine Goehrig, Jean‐Michel Flaman, Sara Jaber, Audrey Griveau, Sophia Djebali, Elisabeth Marcos, Léa Payen, Jacqueline Marvel, Romain Parent, et al.retrieval with citrate buffer. For immunohistological DESMIN staining, the primary antibody diluted 1:200 (ab15200, Abcam) was incubated overnight, before adding the rabbit secondary antibody (BA‐1100, Vector laboratories)

(4) Roles of lipocalin-type and hematopoietic prostaglandin D synthases in mouse retinal angiogenesisJournal of Lipid ResearchOctober 1, 2023Daiki Horikami, Erika Sekihachi, Keisuke Omori, Yui Kobayashi, Koji Kobayashi, Nanae Nagata, Kaori Kurata, Akiyoshi Uemura, Takahisa Muratah, and then incubated overnight at 4°C with primary antibodies; rabbit anti-desmin antibody (1:200; ab15200; Abcam, Cambridge, UK), rabbit anti-fibrinogen antibody (1:500; A0080; Dako, Glostrup, Denmark), rat

(5) DNM2 levels normalization improves muscle phenotypes of a novel mouse model for moderate centronuclear myopathyMolecular Therapy - Nucleic AcidsSeptember 12, 2023Juliana de Carvalho Neves, Foteini Moschovaki-Filippidou, Johann Böhm, Jocelyn LaporteA7811, St. Louis, MO, USA), anti-BIN1 IgG2 (Sigma-Aldrich B9428, St. Louis, MO, USA), anti-desmin (ABCAM AB15200, Cambridge, United Kingdom), anti-DHPR IgG1 (ABCAM ab2862, Cambridge, United Kingdom), anti-DNM2 (homemade

(6) Targeting Endothelial Necroptosis Disrupts Profibrotic Endothelial–Hepatic Stellate Cells Crosstalk to Alleviate Liver Fibrosis in Nonalcoholic SteatohepatitisInternational Journal of Molecular SciencesJuly 11, 2023Mengli Yan, Hui Li, Shiyu Xu, Jinyan Wu, Jiachen Li, Chengju Xiao, Chunheng Mo, Bi-Sen DingCollagen I (Abcam, Waltham, MA, USA; cat: ab34710), α-SMA (Abcam; cat: ab7817), Desmin (Abcam; cat: ab15200) and Lyve1 (Fitzgerald, Dublin, Ireland; cat: 70R-LR003) overnight at 4 °C. Corresponding fluorescent

(7) Transcriptional regulation of Glis2 in hepatic fibrosisExperimental & Molecular MedicineJuly 3, 2023Huan-Yu Gong, Peng-Cheng Zhou, Hao-Ye Zhang, Li-Min Chen, Yang-Mei Zhou, Zhen-Guo Liu(WB); 1:100 (IF); 1:100 (IHC) α-SMA,Abcam,ab7817,1:2000 (WB); 1:100 (IF); 1:100 (IHC) Desmin,Abcam,ab15200,1:1000 (WB); 1:100 (IF); 1:100 (IHC) CoL1A1,Abcam,ab34710,1:1000 (WB); 1:100 (IF) p75NTR,Abcam,ab52987,1:1000

(8) Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient MiceInternational journal of molecular sciencesJune 30, 2023Krzistetzko J, Géraud C, Dormann C, Riedel A, Leibing T.Simple Western" POSTN,"BioTechne, AF2955","IHC, IF, Simple Western" α-SMA,"Abcam, ab5694","IHC, IF" Desmin,"Abcam, ab15200","IHC, IF" F4/80,"BioLegend, 123102","IHC, IF" CD68,"Abcam, ab125212","IHC, IF" CD11b,"BioLegend,

(9) Endothelin Receptor-A Inhibition Decreases Ductular Reaction, Liver Fibrosis, and Angiogenesis in a Model of CholangitisCellular and Molecular Gastroenterology and HepatologyJune 17, 2023Travis Owen, Guido Carpino, Lixian Chen, Debjyoti Kundu, Payton Wills, Burcin Ekser, Paolo Onori, Eugenio Gaudio, Gianfranco Alpini, Heather Francis, et al.1:200","Mouse, Human",Developmental Studies Hybridoma Bank,TROMA-III (Krt19) Desmin,"IF, 1:200",Mouse,Abcam,ab15200 Desmin,IF (Mdk co-stain) 1:20,Mouse,R&D Systems,AF-3844 Endothelin-1 (ET-1),"IF, 1:500 and IHC, 1:200","Mouse,

(10) Reversine attenuates cholestatic ductular reaction in ratsFEBS Open BioMay 1, 2023Di Huang, Lijuan Tang, Tianyang Li, Peilin Li, Zisheng Huang, Jiefeng Weng, Shuai Zhang, Weili Gu, Yu Huangbuffer at 37 °C for 60 min. Tissue sections were incubated overnight at 4 °C with anti‐Desmin (1 : 200, ab15200; Abcam, Guangdong, China), anti‐α‐SMA (1 : 200, ab7817; Abcam), anti‐CK7 (1 : 8000, ab181598; Abcam),

(11) Pericyte-derived cells participate in optic nerve scar formationFrontiers in PhysiologyApril 18, 2023Julia Preishuber-Pflügl, Daniela Mayr, Veronika Altinger, Susanne M. Brunner, Andreas Koller, Christian Runge, Anja-Maria Ladek, Markus Lenzhofer, Francisco J. Rivera, Herbert Tempfer, et al.oligodendrocytes Olig2,Rabbit,Millipore,AB9610,1:250,OPCs and premyelinating oligodendrocytes Desmin,Rabbit,Abcam,ab15200,1:100,Pericytes and vSMCs MBP,Rat,Bio-Rad,MCA409S,1:200,Mature myelinated oligodendrocytes PLP,Rabbit,Santa Cruz

(12) CRISPR-Cas9 editing of a TNPO3 mutation in a muscle cell model of limb-girdle muscular dystrophy type D2Molecular Therapy - Nucleic AcidsMarch 14, 2023Javier Poyatos-García, Águeda Blázquez-Bernal, Marta Selva-Giménez, Ariadna Bargiela, Jorge Espinosa-Espinosa, Rafael P. Vázquez-Manrique, Anne Bigot, Ruben Artero, Juan Jesús Vilchezantibodies at the appropriate dilution. Specific primary antibodies were rabbit anti-Desmin (1:200; ab15200; Abcam, Cambridge, UK) and mouse anti-MHC (1:50; MF-20; Developmental Studies Hybridoma Bank, University

(13) T cell‐dependent bispecific antibodies alter organ‐specific endothelial cell–T cell interactionEMBO reportsMarch 6, 2023Patricia Himmels, Thi Thu Thao Nguyen, Maresa Caunt Mitzner, Alfonso Arrazate, Stacey Yeung, Jeremy Burton, Robyn Clark, Klara Totpal, Raj Jesudason, Angela Yang, et al.anti‐CD3 [SP7], ThermoFisher Scientific RM‐9107‐S—1:100; Pericyte panel [rabbit anti‐Desmin, Abcam ab15200—1:50 + rabbit anti‐NG2, AB5320—1:50 + rabbit anti Smooth Muscle Actin, ThermoFisher Scientific RB9010P—1:50]).

(14) Activating NO-sGC crosstalk in the mouse vascular niche promotes vascular integrity and mitigates acute lung injuryThe Journal of experimental medicineFebruary 6, 2023He H, Yang W, Su N, Zhang C, Dai J, Han F, Singhal M, Bai W, Zhu X, Zhu J, et al.(MA5-15143, 1:100; Thermo Fisher Scientific), rabbit anti-NG2 (ab129051, 1:100); Abcam, rabbit anti-Desmin (Ab15200-1, 1:400; Abcam), rat anti-TER119 (557915, 1:400; Abcam), rat anti-GR1 (108419, 1:200; Biolegend), Hamster

(15) An autocrine signaling circuit in hepatic stellate cells underlies advanced fibrosis in non-alcoholic steatohepatitisScience Translational MedicineJanuary 4, 2023Shuang Wang, Kenneth Li, Eliana Pickholz, Ross Dobie, Kylie P. Matchett, Neil C. Henderson, Chris Carrico, Ian Driver, Martin Borch Borch Jensen, Li Chen, et al.can be found at https://idisco.info/. Antibodies used in this study were DESMIN (Abcam catalog no. ab15200, 1:400 dilution) and CD31 (R&D Systems catalog no. #AF3628, 1:400 dilution). Propidium iodide (Sigma

(16) Rpl3l gene deletion in mice reduces heart weight over timeFrontiers in physiologyJanuary 1, 2023Grimes KM, Prasad V, Huo J, Kuwabara Y, Vanhoutte D, Baldwin TA, Bowers SLK, Johansen AKZ, Sargent MA, Lin SJ, et al.and then applied to the sections overnight at 4°C: HA (BioLegend #901513 at 1:500) and desmin (Abcam, ab15200 at 1:300). The next day, the sections were washed in 1x PBS and then secondary antibodies diluted in

(17) Enzymatically dissociated muscle fibers display rapid dedifferentiation and impaired mitochondrial calcium controliScienceDecember 22, 2022Charlotte Gineste, Sonia Youhanna, Sabine U. Vorrink, Sara Henriksson, Andrés Hernández, Arthur J. Cheng, Thomas Chaillou, Andreas Buttgereit, Dominik Schneidereit, Oliver Friedrich, et al.temperature. After repeated PBS washes, the fibers were incubated with 1:50 rabbit anti-desmin (Abcam; ab15200) or 1:50 rabbit anti-plectin (Huabio; ET1607-80) antibodies overnight at 4°C. The primary antibodies

(18) Role of pericytes in the development of cerebral cavernous malformationsiScienceDecember 22, 2022Dai Z, Li J, Li Y, Wang R, Yan H, Xiong Z, Wu S, Yang X, Lu D, Zhang D, et al.(1:100, Abcam, ab52903), goat anti-KLF4 (1:100, R&D systems, AF3158), rabbit anti-Desmin (1:100, Abcam, ab15200), rabbit anti-Fibrinogen (1:100, ABclonal, A19685), rabbit anti-CD41 (1:100, ABclonal, A11490). Retinal

(19) Cancer cell survival depends on collagen uptake into tumor-associated stromaNat CommunNovember 18, 2022Kuo-Sheng Hsu, James M. Dunleavey, Christopher Szot, Liping Yang, Mary Beth Hilton, Karen Morris, Steven Seaman, Yang Feng, Emily M. Lutz, Robert Koogle, et al.methanol:acetone (50:50) and stained with a chicken anti- GFP (AbCam, ab13970), rabbit anti-desmin (AbCam, ab15200) and rat anti-CD31 (Santa Cruz SC18916). The GFP signal was amplified using FITC goal anti-chicken (Jackson

(20) Expression of Intermediate Filaments in the Developing Testis and Testicular Germ Cell CancerCancers (Basel)November 8, 2022Maria E. Camacho-Moll, Leendert H. J. Looijenga, Roland Donat, Chitranjan J. Shukla, Anne Jørgensen, Rod T. Mitchell19)",mouse,1:3000,1:5000,Mono-clonal,C2562,"Sigma-Aldrich (Poole, UK)" Desmin,rabbit,1:3500,1:3500,Poly-clonal,ab15200,"Abcam Ltd. (Cambridge, UK)" POU5F1,goat,N/A,1:150,Poly-clonal,sc-8626,"Santa Cruz (Heidelberg, Germany)" SOX-9,rabbit,N/A,1:5000,Poly-clonal,A5535,"Merk

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(1)

5/18/2022Great Anti-Desmin Antibody Lucy LiuThis is a nice antibody. We used it as a smooth muscle cell marker and checked expression on Rat pulmonary smooth muscle cells. The signal looks good.

Anti-Desmin antibody - Cytoskeleton Marker from Abcam

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Anti-Desmin antibody - Cytoskeleton Marker from Abcam

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