Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker ab11512 from Abcam

Product Specs
ItemAnti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab11512
Quantity10 UG, 100 UG
ApplicationsImmunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
HostRat
Antibody TypePrimary
ClonalityMonoclonal
CloneDECMA-1
Gene NameCDH1
ReactivityMouse
TargetE Cadherin

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysisSTAR ProtocolsDecember 1, 2023Aikaterini Skorda, Anna Røssberg Lauridsen, Kaisa Huhtinen, Alexandra Lahtinen, Wojciech Senkowski, Jaana Oikkonen, Johanna Hynninen, Sampsa Hautaniemi, Tuula Kallunki"Donkey anti-mouse IgG (H + L), Alexa Fluor 594 (1:1,000)",Thermo Fisher Scientific,Cat# A21203 "E-cadherin (ab11512), rat mAb [DECMA-1] (1:250)",Abcam,Cat# ab11512 "Goat anti-rat IgG (H + L) secondary antibody, Alexa

(2) A Novel Flp Reporter Mouse Shows That TRPA1 Expression Is Largely Limited to Sensory Neuron SubsetseNeuroNovember 21, 2023Mayur J. Patil, Seol-Hee Kim, Parmvir K. Bahia, Sanjay S. Nair, Teresa S. Darcey, Jailene Fiallo, Xiao Xia Zhu, Robert D. Frisina, Stephen H. Hadley, Thomas E. Taylor-ClarkBio,AB_10013483 Anti-RFP,Rabbit,1:1:200,600–401-379,Rockland,AB_2209751 Anti-GFP,Chicken,1:1000,ab13970,Abcam,AB_300798 Anti-GFP,Chicken,1:200,NB100-1614,Novus,AB_10001614 Anti-E-cadherin,Rat,1:300,ab11512,Abcam,AB_298118 Anti-α-smooth muscle actin,Goat,1:300,SAB2500963,MilliporeSigma,AB_10603763 Anti-CGRP,Rabbit,1:300,C8198,MilliporeSigma,AB_259091 Anti-5-HT,Goat,1:600,20079,ImmunoStar,AB_572262 Alexa

(3) Modeling of FAN1-Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid SystemCellsSeptember 20, 2023Lim SW, Na D, Lee H, Fang X, Cui S, Shin YJ, Lee KI, Lee JY, Yang CW, Chung BH.biotinylated Lotus Lectin antibody (LTL; #B-1323;1:100, Vector Laboratories), anti-E-cadherin (ECAD; #ab11512; 1:50, Abcam, Cambridge, UK, #610181; BD Biosciences), anti-podocalyxin (PODXL; #BAF1658; 1:100, R&D Systems

(4) Mesodermal FGF and BMP govern the sequential stages of zebrafish thyroid specificationDevelopmentMay 15, 2023Benoit Haerlingen, Robert Opitz, Isabelle Vandernoot, Angelo Molinaro, Meghna Parakkal Shankar, Pierre Gillotay, Achim Trubiroha, Sabine Costagliola(1:800; PA0300, Biopat Srl), rabbit anti-Nkx2-1 (1:800; PA0100, Biopat Srl), rat anti-E-cadherin (1:300; ab11512, Abcam), Alexa Fluor 488-conjugated donkey anti-rabbit IgG (1:250; 711-545-152, Jackson ImmunoResearch)

(5) Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid systemJournal of translational medicineFebruary 22, 2023Cui S, Fang X, Lee H, Shin YJ, Koh ES, Chung S, Park HS, Lim SW, Lee KI, Lee JY, et al.Cruz Biotechnology), biotinylated Lotus Lectin (LTL, B-1323,1:100; Vector Laboratories), E-Cadherin (ab11512, 1:50; Abcam), CD77 (551352, 1:100; BD Biosciences), and Podocalyxin (BAF1658, 1:100; R&D Systems).

(6) Deep learning predicts the differentiation of kidney organoids derived from human induced pluripotent stem cellsKidney Research and Clinical PracticeJanuary 31, 2023Keonhyeok Park, Jong Young Lee, Soo Young Lee, Iljoo Jeong, Seo-Yeon Park, Jin Won Kim, Sun Ah Nam, Hyung Wook Kim, Yong Kyun Kim, Seungchul Leemicroscope (Carl Zeiss) and ZEN 3.1 software. The following primary antibodies were used: anti-ECAD (1:100, ab11512; Abcam), anti-LTL (1:100, FL‐1321; Vector Labs), and anti-nephrosis 1 (NPHS1) (1:100, AF4269; R&D System). Quantitative real time polymerase chain reaction

(7) Tyk2 is a tumor suppressor in colorectal cancerOncoImmunologyDecember 31, 2022Stefan Moritsch et al.primary antibodies for Ido1 (BioLegend, 654002), MMP7 (Cell Signaling, 3801) or E-cadherin (Abcam, ab11512) in 5% goat serum in PBS. The slides were incubated with fluorochrome-coupled secondary antibodies against

(8) Glucose absorption drives cystogenesis in a human organoid-on-chip model of polycystic kidney diseaseNature CommunicationsDecember 23, 2022Sienna R. Li, Ramila E. Gulieva, Louisa Helms, Nelly M. Cruz, Thomas Vincent, Hongxia Fu, Jonathan Himmelfarb, Benjamin S. Freedman1:5000), ZO-1 (Invitrogen 61-7300, 1:200), Biotinylated LTL (Vector Labs B-1325, 1:500), E-Cadherin (Abcam ab11512, 1:500), SGLT2 (Abcam ab37296, 1:100), laminin-1 (Sigma L9393, 1:50), alpha smooth muscle actin (Sigma

(9) Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic MiceInternational Neurourology JournalSeptember 30, 2022Mi-Hye Kwon, Min-Ji Choi, Fang-Yuan Liu, Fitri Rahma Fridayana, Lashkari Niloofar, Guo Nan Yin, Ji-Kan RyuCat# ab5320), collagen I (Abcam, Cambridge, UK; 1:50; Cat# ab34710), E-cadherin (Abcam; 1:50; Cat# ab11512), tyrosine hydroxylase (TH, Abcam; 1:50; Cat# ab112), choline acetyltransferase (ChAT, Abcam; 1:50;

(10) Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discoveryScience advancesSeptember 23, 2022Hiratsuka K, Miyoshi T, Kroll KT, Gupta NR, Valerius MT, Ferrante T, Yamashita M, Lewis JA, Morizane R.antibodies in ADB overnight at 4°C. The following antibodies were used in these studies: anti-CDH1 (Abcam, ab11512), anti-LTL (Vector Laboratories, B-1325), anti-PODXL (R&D Systems, AF1658), anti-TUBA1A (Abcam, ab179484),

(11) Effective high-throughput isolation of enriched platelets and circulating pro-angiogenic cells to accelerate skin-wound healingCellular and Molecular Life SciencesMay 1, 2022Silvia Erratico et al.mouse monoclonal antibody anti-eNOS (1:100, ab76198, Abcam, UK), rat monoclonal anti-E-cadherin (1:100, ab11512, Abcam, UK), mouse monoclonal anti-cytokeratin 10 (1:100, ab9025, Abcam, UK), rabbit polyclonal anti-loricrin

(12) Kidney Decellularized Extracellular Matrix Enhanced the Vascularization and Maturation of Human Kidney OrganoidsAdvanced ScienceMay 1, 2022Jin Won Kim et al.(Southern Biotech 1310‐01, 1:200), anti‐Collagen IV (Southern Biotech 1340‐01, 1:300), anti‐ E Cadherin (Abcam ab11512, 1:100), anti‐Gb3 (TCI A2506, 1:200), anti‐GLA (Thermo Fisher Scientific PA5‐27349, 1:1000), anti‐Nuclei

(13) Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated ciliaNature biomedical engineeringApril 1, 2022Cruz NM, Reddy R, McFaline-Figueroa JL, Tran C, Fu H, Freedman BS.MAB1368), α-smooth muscle actin (SMA) (R&D MAB1420), ZO-1 (Invitrogen, 339100), E-Cadherin (Abcam, ab11512), Nephrin (R&D, AF4269), p-Histone H3 (Santa Cruz sc-8656-R), OCT4 (Abcam ab19857), brachyury (BRY)

(14) Mapping of the Sensory Innervation of the Mouse Lung by Specific Vagal and Dorsal Root Ganglion Neuronal SubsetseneuroMarch 31, 2022Seol-Hee Kim, Mayur J. Patil, Stephen H. Hadley, Parmvir K. Bahia, Shane G. Butler, Meghana Madaram, Thomas E. Taylor-ClarkBio,AB_10013483 Anti-tdTomato,Rat,1:300,EST203,Kerafast,AB_2732803 Anti-GFP,Chicken,1:1000,ab13970,Abcam,AB_300798 Anti-E-cadherin,Rat,1:300,ab11512,Abcam,AB_298118 Anti-α-smooth muscle actin,Goat,1:300,SAB2500963,MilliporeSigma,AB_10603763 Anti-FLAG,Rabbit,1:300,NB600-345,Novusbio,AB_10001331 Anti-FLAG,Goat,1:300,NB600-344,Novusbio,AB_10000565 Anti-CGRP,Rabbit,1:300,C8198,MilliporeSigma,AB_259091 Neurotrace

(15) Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repairScience translational medicineMarch 2, 2022Gupta N, Matsumoto T, Hiratsuka K, Garcia Saiz E, Galichon P, Miyoshi T, Susa K, Tatsumoto N, Yamashita M, Morizane R.factor α-SMA,Sigma,F3777,1:500 AQP1,Millipore,AB2219,1:200 ATP1A1,Abcam,Ab76020,1:200 cCAS3,Cell signaling,9661S,1:250 CD31,Abcam,ab9498,1:200 CDH1,Abcam,ab11512,1:500 COL1A1,Rockland,600-401-103-0 1,1:200 DESM,Dako,M0760,1:200 FANCD2,Novus biologicals,NB100-182,1:500 FOXD1,LS-Bio,LS-B6453,1:200 Ki67,Dako,m7248,1:200 Ki67,Abcam,Ab15580,1:500 KIM-1,R&D systems

(16) Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signalingBioengineeredMarch 1, 2022Dang L, Shi C, Zhang Q, Liao P, Wang Y.paraformaldehyde for 30 min. Then, the cells were incubated with anti-E-cadherin antibody (cat no. ab11512, Abcam) at 4°C overnight. For Snail staining, the cells were penetrated with Trion-X 100 (Sigma-Aldrich,

(17) m6A-dependent upregulation of TRAF6 by METTL3 is associated with metastatic osteosarcomaJournal of bone oncologyFebruary 1, 2022Wang J, Wang W, Huang X, Cao J, Hou S, Ni X, Peng C, Liu T.After being blocked with 1% BSA, the cells were incubated with primary antibodies to E-cadherin (1:500, ab11512, Abcam), METTL3 (1:300, ab216936, Abcam), Vimentin (1:300, ab193555, Abcam), and TRAF6 (1:1500, ab33915,

(18) Sin3a drives mesenchymal-to-epithelial transition through cooperating with Tet1 in somatic cell reprogrammingStem cell research & therapyJanuary 24, 2022Feng J, Zhu F, Ye D, Zhang Q, Guo X, Du C, Kang J.primary antibodies used in western blotting were anti-Sin3a (Abcam, ab3479), anti-E-cadherin (Abcam, ab11512), anti-β-catenin (Abcam, ab16051), and anti-GAPDH (Bioworld, AP0063). GAPDH was used as the loading

(19) A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the StomachCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Douchi D, Yamamura A, Matsuo J, Lee JW, Nuttonmanit N, Melissa Lim YH, Suda K, Shimura M, Chen S, Pang S, et al.antibodies: rat anti-CD45 (1:500, 14-0451-82; eBioscience, San Diego, CA), rat anti–E-cadherin (1:500, ab11512; Abcam, Cambridge, UK), mouse anti–E-cadherin Alexa Fluor 488/555/647 conjugate (1:200, 560061/560064/560062;

(20) Cross-validation of SARS-CoV-2 responses in kidney organoids and clinical populationsJCI insightDecember 22, 2021Helms L, Marchiano S, Stanaway IB, Hsiang TY, Juliar BA, Saini S, Zhao YT, Khanna A, Menon R, Alakwaa F, et al.antibodies or labels included biotinylated LTL (Vector Laboratories, B-1325; 1:500), E-cadherin (Abcam, ab11512; 1:500), hPODXL (R&D Systems, AF1658; 1:500), ACE2 (antibody 1: R&D Systems, AF933, 1:40; antibody 2:

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Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker from Abcam

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Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker from Abcam

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