Fig 1: Omaveloxolone exerted antioxidative and anti-inflammatory effects on chondrocytes exposed to IL-1β. (A) Representative fluorescence confocal microscopic images of chondrocytes stained with DCFH-DA (scale bar, 100 μm). (B and C) Chondrocytes stained with DCFH-DA were assessed by flow cytometry to quantify the ROS level. (D) Representative Western blots. Quantitative analysis of COX2 (E) and iNOS (F) was performed. (G) The SOD levels in chondrocytes among the four groups. (H) MDA levels in chondrocytes among the four groups. *p < 0.05 and **p < 0.01.
Fig 2: FAS cells up-regulate brain metastasis genes. (a) FAS cells showed enhanced expressions of brain metastasis-related genes. The gene expression was evaluated using qPCR. The statistics were calculated using two-way ANOVA with the post hoc Tukey test. n = 3. (b,c) The expressions of COX2 and SerpinB2 in all groups were tested using immunofluorescence staining. Scale bar = 10 μm. (d,e) Quantification of the fluorescence intensity of COX2 and SerpinB2 in (b,c). The statistics among three groups were calculated based on one-way ANOVA with the post hoc Bonferroni test. n = 50. (ns: no significance, * p < 0.05, *** p < 0.001, **** p < 0.0001).
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