Fig 1: Comparison of Nephrin, WT-1, TNF-a, IL-1ß, eNOS and VEGF protein expression levels of kidney. (A) Nephrin and WT-1 expressions. (B) TNF-a expressions. (C) IL-1ß expressions. (D) eNOS and VEGF expressions. Data are expressed as the mean ± standard deviation (n=6). *P < 0.05, vs the CTL group.
Fig 2: Nephrin, PODXL1, vimentin and a-SMA protein expression levels in the podocytes of diabetic and control rats were examined with western blot analysis. All experiments were performed in triplicate, and the results are presented as the mean ± standard deviation. *P<0.05 vs. control group. PODXL1, podocalyxin-like 1; a-SMA, a-smooth muscle actin.
Fig 3: Comparison of Nephrin, WT-1, TNF-a, IL-1ß, eNOS and VEGF mRNA expression levels of kidney. (A) Nephrin expressions. (B) WT-1 expressions. (C) TNF-a expressions. (D) IL-1ß expressions. (E) eNOS expressions. (F) VEGF expressions. Data are expressed as the mean ± standard deviation (n=6). *P < 0.05, vs the CTL group.
Fig 4: SKP up-regulated the expression of nephrin and podocin. (A) Nephrin expression in glomeruli by immunofluorescence staining. Original magnification ×400. (B) fluorescence intensity of nephrin measured by image J. (C) Western blots for podocin and nephrin expression. (D,E) Quantitative ratios of podocin and nephrin to GADPH. All values are shown as mean ± SEM, with levels of significance determined by ANOVA and a subsequent Tukey test. *** p < 0.001 vs. the indicated groups.
Fig 5: VDR siRNA knocked down VDR reduced the protective effect of aVitD3 on MPC-5 injury Induced by high glucose. MPC-5 cells were transfected with VDR siRNA (si-VDR) or normal control siRNA (si-NC) and subjected to the indicated treatment. (A) Representative image of VDR immunoblot. (B) The MPC-5 cells' viability was determined by the CCK-8 assay. (C) Nephrin, Podocin, Synaptopodin and Desmin expression. Representative images from Western blot results. (D–H) Relative expression of proteins levels. Band densities were measured by the ImageJ program. The ratio of protein/actin density was calculated and normalized with the sham controls. Data were presented as mean ± SD. n = 5. **p < 0.01.
Supplier Page from Abcam for Anti-Nephrin antibody [EPR20993]