Anti-Cytokeratin 15 antibody [LHK15] ab80522 from Abcam

Product Specs
ItemAnti-Cytokeratin 15 antibody [LHK15]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab80522
Quantity10 UG, 100 UG
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Conjugate/TagUnconjugated
HostMouse, Nude mouse
Antibody TypePrimary
ClonalityMonoclonal
CloneLHK15
Gene NameKRT15
ReactivityHuman
TargetCytokeratin 15

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Apoptotic dysregulation mediates stem cell competition and tissue regenerationNature CommunicationsNovember 20, 2023Marianna Yusupova, Roi Ankawa, Yahav Yosefzon, David Meiri, Ido Bachelet, Yaron FuchsCruz, cat. #7943), NFkB p65 (Mouse, 1:100, Santa Cruz #8008), Keratin-15 (Mouse, 1:100, Abcam, cat. #ab80522), and CD34 (Rat, 1:100, Pharmingen, cat. #553731). Antibody staining was visualized using secondary

(2) Single-stage transplantation combined with epidermal stem cells promotes the survival of tissue-engineered skin by inducing early angiogenesisStem cell research & therapyMarch 23, 2023Wang Z, Xu H, Yang H, Zhang Y, Wang X, Wang P, Xu Z, Lv D, Rong Y, Dong Y, et al.cells were incubated with p63 (1:200; ab735; Abcam), ITGα6 (1:200; ab235905; Abcam), and CK15 (1:200; ab80522; Abcam) at 4 °C overnight and then with the corresponding fluorescent secondary antibodies, namely Alexa

(3) Pyruvate Kinase M2 Promotes Hair Regeneration by Connecting Metabolic and Wnt/β-Catenin SignalingPharmaceuticsDecember 13, 2022Yeong Chan Ryu, You-Rin Kim, Jiyeon Park, Sehee Choi, Won-Ji Ryu, Geon-Uk Kim, Eunhwan Kim, Yumi Hwang, Heejene Kim, Gyoonhee Han, et al.USA), rabbit anti-Ki67 (Abcam Cat# ab15580, RRID: AB_443209, 1:500), mouse anti-keratin 15 (Abcam Cat# ab80522, RRID: AB_1603675, 1:200), mouse anti-PCNA (Santa Cruz Technology Cat# sc-56, RRID: AB_628110; 1:500,

(4) Single‐cell transcriptomics provides insights into the origin and microenvironment of human oesophageal high‐grade intraepithelial neoplasiaClinical and Translational MedicineMay 24, 2022Guobin Liao et al.antibodies (Abcam) shown in Table S2. The sections were incubated with antibodies against KRT15 (1:200, ab80522; 1:50, ab52816), KRT19 (1:200, ab52625), STMN1 (1:2000, ab52630) and KRT13 (1:2000, ab16112). The sections

(5) Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendagesBioengineering & translational medicineMay 1, 2021Abreu CM, Gasperini L, Lago MEL, Reis RL, Marques AP.PRB‐155P, BioLegend), Involucrin (1:25, ab68, Abcam), fibronectin (1:100, ab2413, Abcam), K15 (1:50, ab80522, Abcam), β‐catenin (1:100, 610,154, BD Transduction Laboratories) and α‐SMA (1:200, ab7817, Abcam) was

(6) The common YAP activation mediates corneal epithelial regeneration and repair with different-sized woundsNPJ Regenerative medicineMarch 26, 2021Li Y, Ge L, Chen X, Mao Y, Gu X, Ren B, Zeng Y, Chen M, Chen S, Liu J, et al.1:2000), rabbit p63α (Abcam, ab124762; 1:2000), rabbit CK14 (Abcam, ab181595; 1:2000), mouse CK15 (Abcam, ab80522; 1:3000), rabbit Ki67 (Abcam, ab15580; 1:800), rabbit p-Cofilin (Ser3; Abcam, ab12866; 1:2000), rabbit

(7) Rat epidermal stem cells promote the angiogenesis of full-thickness woundsStem Cell Research & TherapyDecember 1, 2020Shaobin Huang et al.(1:200; ab735; Abcam), α6-integrin (1:200; ab235905; Abcam), CD71 (1:200; ab22391; Abcam), CK15 (1:200; ab80522; Abcam), CK19 (1:200; ab84632; Abcam), CD31 (1:200; ab24590; Abcam), and CD34 (1:200; ab81289; Abcam).

(8) A Novel Human Skin Tissue Model To Study Varicella-Zoster Virus and Human CytomegalovirusJournal of VirologyOctober 27, 2020Megan G. Lloyd, Nicholas A. Smith, Michael Tighe, Kelsey L. Travis, Dongmei Liu, Prashant K. Upadhyaya, Paul R. Kinchington, Gary C. Chan, Jennifer F. MoffatAntibodies used were vimentin (AB194719; Alexa Flour 647; 1:200; Abcam), cytokeratin 15 primary antibody (AB80522; 1:100; Abcam), CD45 (number 562279; 1:100; BD), CD34 (AB81289; 1:50; Abcam), and Hoechst 33342 (number

(9) Immunohistochemistry of Canine Hair Follicle Stem Cells (cHFSCs) by using CK15 and CK19Journal of Animal ResearchApril 25, 2020N. Hemavathi,blocking agent for 2 hours. Sections were incubated with primary antibody Anti-Cytokeratin 15 (Cat. No. ab80522, Abcam) at 1.25µg/ml concentration in 1% BSA for overnight at 4°C. Thensections werepermeabilized with

(10) Osmolyte transporter expression is reduced in photoaged human skin: Implications for skin hydration in agingAging CellJanuary 1, 2020April R. Foster, Cecile El Chami, Catherine A. O'Neill, Rachel E. B. Watsonanti‐e‐cadherin (dilution 1:500; catalogue #ab1416; Abcam), mouse anti‐K15 (dilution 1:1,000; catalogue #ab80522; Abcam), mouse anti‐CD31 (dilution 1:100; catalogue #M0823; Dako), and mouse anti‐vimentin (dilution

(11) Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle DevelopmentTheranosticsJanuary 1, 2020Ge W, Tan SJ, Wang SH, Li L, Sun XF, Shen W, Wang X.polyclonal anti-SHH,Proteintech,"Cat# 20697-1-AP, RRID:AB_10694828" Mouse monoclonal anti-K15,Abcam,"Cat# ab80522, RRID:AB_1603675" Rabbit monoclonal anti-BMP2,Abcam,Cat# ab214821; RRID:N/A Mouse monoclonal anti-PCNA,Santa Cruz

(12) The Potential Role of Cycloastragenol in Promoting Diabetic Wound Repair In VitroBioMed Research InternationalDecember 20, 2019Yi Cao, Li Xu, Xiaohong Yang, Yuan Dong, Hongbin Luo, Fengling Xing, Qiongxiang GeFlow cytometry and immunofluorescence (IF) staining with antibodies of K19 (1 : 200 diluted, ab52625, Abcam, UK), K15 (1 : 100 diluted, ab80522, Abcam), K14 (1 : 200 diluted, ab9220, Abcam), CD34 (1 : 200 diluted, ab81289, Abcam), and β1-integrin

(13) Pre-aggregation of scalp progenitor dermal and epidermal stem cells activates the WNT pathway and promotes hair follicle formation in in vitro and in vivo systemsStem Cell Research & TherapyDecember 19, 2019Yiqun Su, Jie Wen, Junrong Zhu, Zhiwei Xie, Chang Liu, Chuan Ma, Qun Zhang, Xin Xu, Xunwei Wuanti-cytokeratin 14 (K14) (Cat. Ab119695, Abcam); monoclonal mouse anti-cytokeratin 15 (K15) (Cat. ab80522, Abcam); monoclonal rabbit anti-cytokeratin 17 (K17) (Cat. ab51056, Abcam); monoclonal rabbit anti-cytokeratin 6

(14) Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membraneStem Cell Research & TherapyDecember 1, 2019Huateng Zhou, Lixiang Wang, Cui Zhang, Jintao Hu, Jianlin Chen, Weibin Du, Fei Liu, Weifan Ren, Jinfu Wang, Renfu Quangoat serum for 10 min and then incubated with a mouse polyclonal anti-Krt15-specific antibody (1:50, ab80522), anti-CK10-specific antibody (1:200, ab76318), and anti-loricrin-specific antibody (1:200, ab85679)

(15) The monoclonal antibody EPR1614Y against the stem cell biomarker keratin K15 lacks specificity and reacts with other keratinsScientific ReportsDecember 1, 2019Hebah Aldehlawi, Katarzyna A. Niemiec, Deepa R. Avisetti, Anand Lalli, Muy-Teck Teh, Ahmad Waseemprimary antibodies used in this study were as follows: anti-GAPDH (ab9485), mouse anti-K15 clone LHK15 (ab80522), rabbit anti-K15 clone EPR1614Y (ab52816) and anti-K10 clone RKSE60 (ab902) from Abcam, UK. The secondary

(16) Fibronectin precoating wound bed enhances the therapeutic effects of autologous epidermal basal cell suspension for full-thickness wounds by improving epidermal stem cells’ utilizationStem Cell Research & TherapyDecember 1, 2019Peng Wang et al.37 °C. For double labeling, two compatible primary anti-rats antibodies were added: anti-K15 (1:250, ab80522; Abcam) and anti-Ki-67 (1:200, ab16667; Abcam). After incubating at 4 °C overnight, the sections were

(17) CDK4/6 inhibition mitigates stem cell damage in a novel model for taxane‐induced alopeciaEMBO Molecular MedicineOctober 1, 2019Talveen S Purba et al.(1:100), Cleaved Caspase‐3 (Asp175) (Cell signalling #9661) (1:50), Anti‐Cytokeratin 15 [LHK15] (Abcam, ab80522) (1:500), Anti‐Cytokeratin 15 [EPR1614Y] (Abcam, ab52816) (1:500). Goat anti‐Mouse/Rabbit Secondary

(18) Mutant Lef1 controls Gata6 in sebaceous gland development and cancerThe EMBO journalMay 2, 2019Oulès B, Rognoni E, Hoste E, Goss G, Fiehler R, Natsuga K, Quist S, Mentink R, Donati G, Watt FM.at the indicated dilutions: Fabp5 (1:100, R&D Systems AF1476); Krt15 (1:1,000, LHK‐15 clone, Abcam ab80522); Lef1 (1:100–500, C12A5 clone, Cell Signaling 2230 and 8490); Gata6 (1:100–1,000, D61E4 clone, Cell

(19) Intersections between Regulated Cell Death and AutophagyTrends in cell biologyApril 1, 2019Napoletano F, Baron O, Vandenabeele P, Mollereau B, Fanto M.at the indicated dilutions: Fabp5 (1:100, R&D Systems AF1476); Krt15 (1:1,000, LHK-15 clone, Abcam ab80522); Lef1 (1:100–500, C12A5 clone, Cell Signaling 2230 and 8490); Gata6 (1:100–1,000, D61E4 clone, Cell

(20) In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell IsolatesFrontiers in bioengineering and biotechnologyJanuary 1, 2019Yang Q, Pinto VMR, Duan W, Paxton EE, Dessauer JH, Ryan W, Lopez MJ.Human Mouse Abcam Inc Ab7800 PBS K15 N/A Keratin 15 Human, Cow, Rat, Mouse Mouse Abcam Inc Ab80522 PBS K19 MAP2 IgG IgG IgG IgG DSG1 DSG3 N/A N/A FITC Alexa Fluor 633 Alexa Fluor 488 Alexa Fluor 594 N/A N/A Keratin

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Anti-Cytokeratin 15 antibody [LHK15] from Abcam

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Anti-Cytokeratin 15 antibody [LHK15] from Abcam

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