Fig 1: Single-cell time course analysis(A) Experimental strategy. Image created with Biorender.(B) UMAP of time course scRNA-seq data during differentiation of naive hPSCs under ASECRiAV condition as well as day 70 EXMCs, colored by days.(C) As in (B), colored by cell types.(D) Expression of selected markers in (B).(E) Proportion of cell types during ASECRiAV conversion in (B). Cell numbers can be found in Table S5.(F) Expression of selected genes during conversion.(G) IF for the indicated markers during naive hPSC to ASECRiAV conversion. Scale bar 100 µm. Please note feeders have background staining for NR2F2 in D0-D4 images.(H) Quantification of (G). n = two rounds of differentiation. See also Figure S5 and Table S5.
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