Fig 2: Phenotypic differences during lung myeloid DC maturation. Analysis of cell surface CCR7 and CD83 expression in myeloid DC maturation in the different groups (n=8 each). Representative histograms for each group are shown. Isotype controls are presented as grey-shaded areas and specific antibodies are shown as black lines. CD, cluster of differentiation; CCR, chemokine receptor; CS, cigarette smoker; COPD, chronic obstructive pulmonary disease; CS-COPD-, non-smoker without COPD; CS-COPD+, non-smoker with COPD; CS+COPD-, cigarette smoker without COPD; CS+COPD+, cigarette smoker with COPD.

Fig 3: CD83+, CD1a+ and CCR7+ expression levels in lung tissues from the different groups. The results are presented as the mean ± SD, n=8, *P<0.05, **P<0.01, as compared with the control for each marker at a baseline of 1. CD, cluster of differentiation; CCR, chemokine receptor; CS, cigarette smoker; COPD, chronic obstructive pulmonary disease; CS-COPD-, non-smoker without COPD; CS-COPD+, non-smoker with COPD; CS+COPD-, cigarette smoker without COPD; CS+COPD+, cigarette smoker with COPD.

Fig 4: Immunohistochemical analysis of CD83+, CD1a+ and CCR7+ cells in the following groups: (A) CS-COPD-; (B) CS-COPD+; (C) CS+COPD- and (D) CS+COPD+. Magnification, ×400. Arrows indicate CD83+, CD1A+ and CCR7+ stained cells. CD, cluster of differentiation; CCR, chemokine receptor; CS, cigarette smoker; COPD, chronic obstructive pulmonary disease; CS-COPD-, non-smoker without COPD; CS-COPD+, non-smoker with COPD; CS+COPD-, cigarette smoker without COPD; CS+COPD+, cigarette smoker with COPD.