Fig 2: Flow diagram of patients included in the study. Gas6, growth arrest-specific gene 6; VTE, venous thromboembolism.

Fig 3: Inflammatory stimuli associated with viral infections upregulate Axl and promote Gas6 binding to macrophages. (A–E) MDMs were stimulated for 24 h with dexamethasone (Dex), LPS, IFN-?, poly(I:C) or IFN-a. (A) MerTK and (C) Axl protein expression determined by ELISA of total cell lysates (mean+SEM, n = 4). (B) MerTK and (D) Axl protein expression analyzed by western blotting; actin was used as loading control. A representative of two to three independent experiments is shown. (E) Flow cytometric analysis of Axl expression on MDMs stimulated with poly(I:C) or IFN-a for 24 h. Representative histograms of two independent experiments are shown; dotted line/shaded: FMO control. (F) Axl and MerTK relative mRNA and (G) protein expression in MDMs differentiated by 6-day culture in M-CSF or GM-CSF determined by qPCR (mean+SEM, n = 6) and by ELISA of total cell lysates (mean+SEM, n = 3), respectively. (H) Axl, MerTK and Tyro3 relative mRNA expression in MDMs stimulated as in (A–C) analyzed by qPCR (mean+SEM, n = 2–3). (I and J) Flow cytometric analysis of Gas6 binding to MDMs stimulated with poly(I:C) for 24 h. (I) Representative of four independent experiments is shown; dotted line/shaded: isotype control. (J) Ratio of Gas6 to isotype control geometric mean fluorescence intensity (MFI)+SEM in unstimulated and poly(I:C)-stimulated MDMs (n = 4). *p < 0.01, paired t-test. ELISA and qPCR samples were assayed in duplicate.

Fig 4: Cumulative incidence rates of venous thromboembolism (VTE), major bleeding and mortality for strata of growth arrest-specific gene 6 (Gas6). The cumulative incidence rates of VTE (A), major bleeding (B) and mortality (C) for strata of Gas6 levels were estimated with the Kaplan–Meier method, and survivor functions across groups were compared by use of the log-rank test. Gas6 levels were categorized on the basis of the lower and upper quartiles as low (< 109%), medium (109–157%), and high (> 157%).