Fig 2: Effect of fruit extract on LDLR and PCSK9 expression in Huh7 cells. A) and B) LDLR and PCSK9 expression was evaluated by western blot analysis. GAPDH was used as a loading control. Densitometric readings were evaluated using the ImageLab™ software, and the relative intensity of the bands are shown in the histograms. C) and D) LDLR and PCSK9 mRNA expression was evaluated by RT-qPCR analysis and normalized with 18S. *p < 0.01; **p < 0.01 vs Ctr. Ctr: control; S: simvastatin (5 µM).

Fig 3: Effect of leaves EtAc and ButOH extracts on LDLR and PCSK9 expression in Huh7 cell line. A) and B) LDLR and PCSK9 expression was evaluated by western blot analysis. GAPDH was used as a loading control. Densitometric readings were evaluated using the ImageLab™ software, and the relative intensity of the bands are shown in the histograms. C and D) LDLR and PCSK9 mRNA expression was evaluated by RT-qPCR analysis and normalized with 18S. *p < 0.01; **p < 0.01; ***p < 0.001 vs Ctr. Ctr: control; S: simvastatin (5 µM).