Fig 1: SAV1 phosphorylation by STK25 inhibits SAV1-dependent activation of MST1/2.(A and B) Immunoblots and quantification of pT180 levels of lysates of 293FT cells co-transfected with FLAG-MST2 and the indicated plasmids. The relative anti-pT180 intensities were normalized to MST2. Data are plotted as mean ± SEM of three biological replicates (****p<0.0001; **p<0.01; ns, non-significant). (C) 293FT cells were transfected with the indicated FLAG-SAV1 plasmids. The total cell lysates (input) and anti-FLAG IP were blotted with the indicated antibodies. (D) Model for regulation of SAV1-STRIPAK antagonism by STK25.