CD4 Antibody AF554 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF554
NCBI Gene AliasesL3T4, Ly-4
ApplicationsWestern Blot
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
ReactivityMouse
IsotypeIgG
TargetCD4
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene NameCD4 antigen
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CD4, Lys27-Thr394, Accession # P06332
ConcentrationLYOPH
Brand/TrademarkR&D Systems, a Bio-Techne Brand
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects mouse CD4 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant human CD4, recombinant feline CD4, and recombinant canine CD4 is observed

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Soluble RAGE Prevents Type 1 Diabetes Expanding Functional Regulatory T CellsDiabetesSeptember 1, 2022Sherman S. Leung, Danielle J. Borg, Domenica A. McCarthy, Tamar E. Boursalian, Justen Cracraft, Aowen Zhuang, Amelia K. Fotheringham, Nicole Flemming, Thomas Watkins, John J. Miles, et al.blocking with 10% donkey serum, and incubation with rabbit anti-CD3 (SP7; Abcam), goat anti-CD4 (no. AF554; R&D Systems), and biotinylated anti-FoxP3 (FJK-16s; eBioscience) antibodies overnight at 4°C. This

(2) LDH-A—Modulation and the Variability of LDH Isoenzyme Profiles in Murine Gliomas: A Link with Metabolic and Growth ResponsesCancers (Basel)May 6, 2022Masahiro Shindo, Masatomo Maeda, Ko Myat, Mayuresh M. Mane, Ivan J. Cohen, Kiranmayi Vemuri, Avi S. Albeg, Inna Serganova, Ronald Blasbergstained with immune markers: anti-CD68 antibody (Catalog No. TA1518, Boster), anti-CD4 (Catalog No. AF554, R & D Systems) and anti-CD3 antibody (Catalog No. A0452, Dako). Quantification of morphological characteristics

(3) Kidney disease risk factors do not explain impacts of low dietary protein on kidney function and structureiScienceNovember 19, 2021Fotheringham AK, Solon-Biet SM, Bielefeldt-Ohmann H, McCarthy DA, McMahon AC, Ruohonen K, Li I, Sullivan MA, Whiddett RO, Borg DJ, et al.#ab16669, clone SP7 -, Abcam, Cambridge, The United Kingdom), anti-CD4 goat polyclonal IgG (10 μg/ml, #AF554 R&D Systems, Minneapolis, USA) and anti-FoxP3 biotinylated rat monoclonal IgG (5 μg/ml, # 13-5773-82,

(4) Selective Inhibition of HDAC3 Targets Synthetic Vulnerabilities and Activates Immune Surveillance in LymphomaCancer discoveryMarch 1, 2020Mondello P, Tadros S, Teater M, Fontan L, Chang AY, Jain N, Yang H, Singh S, Ying HY, Chu CS, et al.Memorial Sloan Kettering Cancer Center. Sections were stained with either anti- CD4 (R&D Systems, cat#AF554, 2ug/ml) or anti-PD-L1 (R&D Systems cat#AF1019, 2ug/ml) or B220 (BD Biosciences, cat# 550286, 0.3ug/ml)

(5) The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune SuppressionCancer cellAugust 12, 2019Su W, Han HH, Wang Y, Zhang B, Zhou B, Cheng Y, Rumandla A, Gurrapu S, Chakraborty G, Su J, et al.550286, 0.3 μg/ml); anti-FoxP3 (eBioscience, cat# 14–577382, 5 μg/ml); anti-CD4 (R&D Systems, cat# AF554, 2 μg/ml); anti-NKp46 (R&D Systems, cat#AF2225, 2 μg/ml) for 5 hours, followed by 60 minutes incubation

(6) Non-conventional Inhibitory CD4⁺Foxp3^-PD-1^hi T Cells as a Biomarker of Immune Checkpoint Blockade ActivityCancer cellJune 11, 2018Zappasodi R, Budhu S, Hellmann MD, Postow MA, Senbabaoglu Y, Manne S, Gasmi B, Liu C, Zhong H, Li Y, et al.anti-mouse IL-21 (clone FFA21),eBioscience,Cat# 17-7211-82 Purified anti-mouse CD4,R&D Systems,Cat# AF554 Purified anti-mouse Foxp3 (clone FJK-16s),eBioscience,Cat# 14-5773-82 Purified anti-mouse PD-1,Sino Biological

(7) Diphtheria toxin-mediated ablation of lymphatic endothelial cells results in progressive lymphedemaJCI insightSeptember 22, 2016Gardenier JC, Hespe GE, Kataru RP, Savetsky IL, Torrisi JS, Nores GD, Dayan JJ, Chang D, Zampell J, Martínez-Corral I, et al.and insight.jci.org doi:10.1172/jci.insight.84095 11 RESEARCH ARTICLE CD4 (catalog AF2125, MAB114, and AF554, respectively; R&D Systems), α-SMA (catalog C6198; Sigma-Aldrich), CD11b (catalog 557395; BD Biosciences),

(8) Determinants in the β and δ subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptorThe Journal of biological chemistryJanuary 3, 2014Rudell JC, Borges LS, Rudell JB, Beck KA, Ferns MJ.

(9) Timing of estrogen replacement influences atherosclerosis progression and plaque leukocyte populations in ApoE-/- miceAtherosclerosisNovember 1, 2008Cann JA, Register TC, Adams MR, St Clair RW, Espeland MA, Williams JK.

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CD4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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CD4 Antibody from R&D Systems, a Bio-Techne Brand

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