CD4 Antibody AF-379-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD4 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF-379-NA
NCBI Gene AliasesCD4mut, Leu-3, IMD79, T4, OKT4D
ApplicationsWestern Blot, Simple Western, Immunohistochemistry, Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
ReactivityHuman
IsotypeIgG
Quantity100 ug (also 25 ug)
TargetCD4
HostGoat
NCBI Full Gene NameCD4 molecule
ClonalityPolyclonal
ImmunogenS. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4, Lys26-Trp390, Accession # P01730
ConcentrationLYOPH
PurityAntigen Affinity-purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human CD4 in direct ELISAs and Western blots

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) AZD5582 plus SIV-specific antibodies reduce lymph node viral reservoirs in antiretroviral therapy-suppressed macaquesNature medicineOctober 1, 2023Dashti A, Sukkestad S, Horner AM, Neja M, Siddiqi Z, Waller C, Goldy J, Monroe D, Lin A, Schoof N, et al.HNBP2-34039, Novus), mouse monoclonal anti-CD20 (1:500, M0755, DAKO) and goat polyclonal anti-CD4 (1:1,000, AF-379-NA, R&D Systems). Slides were washed and then incubated with secondary donkey anti-goat IgG-Alexa Fluor

(2) Tertiary lymphoid structures correlate with enhancement of antitumor immunity in esophageal squamous cell carcinomaBritish journal of cancerOctober 1, 2023Nakamura S, Ohuchida K, Hayashi M, Katayama N, Tsutsumi C, Yamada Y, Hisano K, Okuda S, Ohtsubo Y, Iwamoto C, et al.

(3) Polyclonal lymphoid expansion drives paraneoplastic autoimmunity in neuroblastomaCell ReportsAugust 2, 2023Miriam I. Rosenberg, Erez Greenstein, Martin Buchkovich, Ayelet Peres, Eric Santoni-Rugiu, Lei Yang, Martin Mikl, Zalman Vaksman, David L. Gibbs, Dan Reshef, et al.incubated with the slides for 1 h at room temperature, and b) Goat anti-human CD4 (1:400, R&D Systems AF-379-NA) and Rabbit anti-human CD8 (1:400, Thermo RB-9009-P0), which were both incubated for 1 h at room temperature.

(4) Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) modelExperimental & molecular medicineAugust 1, 2023Lee AR, Lee SY, Choi JW, Um IG, Na HS, Lee JH, Cho ML.Paraffin-embedded sections were incubated with 10% normal goat serum for 30 min and stained with anti-CD4 (AF-379-NA; R&D Systems), anti-IL-17 (MAB3171-100; R&D Systems), anti-IL-4 (PA5-25165; Thermo Fisher Scientific),

(5) Step-dose IL-7 treatment promotes systemic expansion of T cells and alters immune cell landscape in blood and lymph nodesiScienceFebruary 17, 2023Pandit H, Valentin A, Angel M, Deleage C, Bergamaschi C, Bear J, Sowder R, Felber BK, Pavlakis GN.for Tfh Ki67+ quantification IFA was performed by combining ON goat anti-CD4 (R&D system, 1:1000 cat#AF-379-NA), rabbit anti-KI67 (Thermo science, 1:200 cat# MA5-14520) and mouse anti-BCL6 (DAKO, 1:100) in TBS-tween.

(6) Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal AdenocarcinomaJournal of personalized medicineApril 13, 2022Haddad R, Zlotnik O, Goshen-Lago T, Levi M, Brook E, Brenner B, Kundel Y, Ben-Aharon I, Kashtan H.

(7) E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune ResponsesVaccinesFebruary 15, 2022Sangare K, Helmold Hait S, Moore M, Hogge C, Hoang T, Rahman MA, Venzon DJ, LaBranche C, Montefiori D, Robert-Guroff M, et al.(Cat# 5420, Advanced Bioscience Laboratories, Inc. (ABL), Rockville, MD, USA); goat anti-hCD4 IgG (Cat# AF-379-NA, R&D Systems); mouse anti-DNA binding protein (DBP) (gift from David Ornelles, Wake Forest University,

(8) Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signalingiScienceJanuary 21, 2022Akusjärvi SS, Ambikan AT, Krishnan S, Gupta S, Sperk M, Végvári Á, Mikaeloff F, Healy K, Vesterbacka J, Nowak P, et al.min at RT using 4% PFA. Cells were blocked using 3% BSA and antibody detection of CD4 (R&D systems, #AF-379-NA) and Alexa 647 conjugated CD8 (Abcam, #ab196193) performed prior permeabilization 10 min using 0.2%

(9) Microbial Protein Binding to gC1qR Drives PLA2G1B-Induced CD4 T-Cell AnergyFrontiers in immunologyJanuary 1, 2022Pothlichet J, Meola A, Bugault F, Jeammet L, Savitt AG, Ghebrehiwet B, Touqui L, Pouletty P, Fiore F, Sauvanet A, et al.stained using anti-human CD4 (mouse anti-CD4 clone RPA-T4, 555344, BD Biosciences; or goat anti-CD4, AF-379-NA, R&D/Novus), followed by donkey anti-mouse-AlexaFluor488 (A21202, Thermofisher). Phosphorylation of

(10) Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity LymphedemaBiologySeptember 18, 2021Mehrara BJ, Park HJ, Kataru RP, Bromberg J, Coriddi M, Baik JE, Shin J, Li C, Cavalli MR, Encarnacion EM, et al.

(11) Increased Circulating T Follicular Helper Cells Induced via IL-12/21 in Patients With Acute on Chronic Hepatitis B Liver FailureFrontiers in ImmunologyMarch 31, 2021Bingying Du, Jiaming Teng, Rongkun Yin, Yuanyuan Tian, Tingwang Jiang, Yanan Du, Wei Caiantibodies against rabbit anti-human CXCR5 (Abcam, #ab133706) and goat anti-human CD4 (R&D systems, #AF-379-NA), followed by Alexa Fluor 488 or 555 conjugated secondary antibodies (Invitrogen, Carlsbad, CA). The

(12) Distribution of Immune Cells Including Macrophages in the Human CochleaFrontiers in neurologyJanuary 1, 2021Liu W, Danckwardt-Lillieström N, Schrott-Fischer A, Glueckert R, Rask-Andersen H.R",1:100,14-0194-82,Thermo Fisher Scientific CD8α,M,Mouse,H,1:100,MAB1509,RandD Systems CD4,P,Goat,H,1:150,AF-379-NA,RandD Systems CD68,M,Mouse,"H, M, R",1:50,NB100-683,Novus TLR4,OC,Rabbit,"H, M",1:10,710185,Thermo Fisher Scientific

(13) PLA2G1B is involved in CD4 anergy and CD4 lymphopenia in HIV-infected patientsThe Journal of clinical investigationJune 1, 2020Pothlichet J, Rose T, Bugault F, Jeammet L, Meola A, Haouz A, Saul F, Geny D, Alcami J, Ruiz-Mateos E, et al.respectively anti– human CD4 (mouse anti-CD4 clone RPA-T4, 555344, BD Biosciences; or goat anti-CD4, AF-379-NA, R&D Systems/Novus) and anti–human CD8 (mouse anti-CD8 clone RPA-T8, 555364, BD Biosciences), labeled

(14) Intra- and Inter-cellular Rewiring of the Human Colon during Ulcerative ColitisCellJuly 25, 2019Smillie CS, Biton M, Ordovas-Montanes J, Sullivan KM, Burgin G, Graham DB, Herbst RH, Rogel N, Slyper M, Waldman J, et al.ThermoFisher MA1–25914), mouse anti-CD11c (1:100, BD Biosciences 550375), goat anti-CD4 (1:100, R&D Systems AF-379-NA), rabbit anti-CD8 (1:100, Invitrogen SP16). Alexa Fluor 488-, 594-, and 647-conjugated secondary antibodies

(15) A Map of Human Type 1 Diabetes Progression by Imaging Mass CytometryCell metabolismMarch 5, 2019Damond N, Engler S, Zanotelli VRT, Schapiro D, Wasserfall CH, Kusmartseva I, Nick HS, Thorel F, Herrera PL, Atkinson MA, et al.anti-CD3ε,Cell Signaling Technology,"Cat#85061, RRID:AB_2721019" Goat polyclonal anti-CD4,R and D Systems,"Cat#AF-379-NA, RRID:AB_354469" Mouse monoclonal anti-CD8a,Thermo Fisher Scientific,"Cat#14–0085-82, RRID:AB_11150240" Mouse

(16) Lymphoid Aggregates in the CNS of Progressive Multiple Sclerosis Patients Lack Regulatory T CellsFrontiers in immunologyJanuary 1, 2019Bell L, Lenhart A, Rosenwald A, Monoranu CM, Berberich-Siebelt F.(1:200, Dako, #M0755), CD3 (1:100, Dako, #A0452), CD3 (1:50, abcam, #11089), CD4 (1:200, R&D Systems #AF-379-NA), CD8 (1:100, Dako #M7103), CD27 (1:50, Sigma-Aldrich, #HPA038936), CD138 (1:200, BioLegend, # 356502),

(17) Human Inner Ear Immune Activity: A Super-Resolution Immunohistochemistry StudyFrontiers in neurologyJanuary 1, 2019Liu W, Kämpfe Nordström C, Danckwardt-Lillieström N, Rask-Andersen H.MN, USA CD11b Monoclonal 1:50 Rabbit AB52478 Abcam, Cambridge, UK CD4 Polyclonal 1:150 Goat AF-379-NA R&D Systems CD8α Monoclonal 1:100 Mouse MAB1509 R&D Systems CD68 Monoclonal 1:50 Mouse NB100-683 Novus,

(18) Persistence of a T Cell Infiltrate in Human Ganglia Years After Herpes Zoster and During Post-herpetic NeuralgiaFrontiers in microbiologyJanuary 1, 2019Sutherland JP, Steain M, Buckland ME, Rodriguez M, Cunningham AL, Slobedman B, Abendroth A.

(19) Distinct Inflammatory Profiles in Atopic and Nonatopic Patients With Chronic Rhinosinustis Accompanied by Nasal Polyps in Western ChinaAllergy, Asthma & Immunology ResearchApril 21, 2015Luo Ba, Jintao Du, Feng Liu, Fenglin Yang, Miaomiao Han, Sixi Liu, Ping Lin, Huabin Liby immunohistochemistry. Briefly, air-dried sections were incubated with 1% anti-human CD4 antibody (AF-379-NA, R&D Systems, Minneapolis, MN, USA) or 1% mouse monoclonal antibodies CD20 (clone L26; Dako, Carpinteria,

(20) Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosisThe Journal of clinical investigationFebruary 1, 2015Reissfelder C, Stamova S, Gossmann C, Braun M, Bonertz A, Walliczek U, Grimm M, Rahbari NN, Koch M, Saadati M, et al.with primary antibodies against human CD4 (polyclonal goat anti-human CD4, R&D Systems, catalog no. AF-379-NA, 1:10) and human FOXP3 (monoclonal mouse anti-human FOXP3; AbD Serotec MCA2376, 1:100), followed by

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CD4 Antibody

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CD4 Antibody

CD4 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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CD4 Antibody from R&D Systems, a Bio-Techne Brand

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