Fig 1: Confirmation of S100A9, p62, COL17A1, and LC3 expression in olaparib resistant cells. (A) Western blot analysis of S100A9, p62, COL17A1, and p62 associated protein LC3-I, II in both parental and resistant SUM159 and MDA468 cells. (B) Effect of autophagy stimulation on p62 and LC3 expression. Parental and resistant SUM159 cells were starved for indicated periods (upper panel) or treated with two different doses (5 µM and 10 µM) of rapamycin for 24 hrs (lower panel). The levels of p62 or LC3 were measured by western blotting. Rap, rapamycin and starv, starvation. (C) Effect of olaparib withdrawal on LC3 and p62 levels. SUM159-R cells were maintained in olaparib-free media for 3 days and 10 days or in olaparib-containing (25 µM) medium (ola).). P, parental; R, resistant; Starv., starvation; Rap, rapamycin; and Ola, olaparib.