Fig 2: Schematic representation of the human OCT4 gene. List of transcript variants for OCT4. The OCT4A transcript variant is composed of exon 1a, and exons 2 to 5. The OCT4B transcript variant contains exon 1b, and exons 2, 3, 4 and 5. The OCT4B1 transcript variant contains exon 1b, exon 2, exon 2b, exon 3, 4 and 5. The OCT4B2 transcript variant is composed of exon 1b lacking a 630-nt fragment, 2, 2b, 3, 4 and 5. The OCT4B3 transcript variant is composed of exons 1b lacking a 207-nt fragment, exon 2, 2b, 3, 4 and 5. The OCTB4 transcript variant is composed of exon 1b, The OCT4B transcript variant contains exon 1b, exon 2 lacking a 28-nt fragment, exons 3, 4 and 5.

Fig 3: Specificity of various antibodies against OCT4A. Immunofluorescent cell staining showing expression of OCT4 in hESCs (positive control), MG63 (negative control), 293T, osteoblast-differentiated hAFSCs, HeLa cells and HepG2 cells. Nuclei were stained with DAPI (blue). Scale bar 50 µm.