Purified (azide-free) anti-Neurofilament H (NF-H), Nonphosphorylated 801702 from BioLegend

Product Specs
ItemPurified (azide-free) anti-Neurofilament H (NF-H), Nonphosphorylated
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number801702
Quantity25 µL
ApplicationsImmunohistochemistry (IHC)-P, Western Blot (WB), Array Tomography, Immunocytochemistry (ICC), SB
Concentration1.00 mg/mL
Conjugate/TagUnconjugatedAF
FormatPhosphate-buffered solution (no preservatives or carrier proteins)
ReactivityHuman, Mouse, Rat
TargetNeurofilament H
IsotypeIgG1
CloneSMI 32
HostMouse
NCBI Full Gene Nameneurofilament heavy chain
NCBI Gene AliasesCMT2CC, NFH
ClonalityMonoclonal

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Spatial transcriptomics reveals molecular dysfunction associated with Lewy pathologybioRxivMay 20, 2023Thomas Goralski, Lindsay Meyerdirk, Libby Breton, Laura Brasseur, Kevin Kurgat, Daniella DeWeerd, Lisa Turner, Katelyn Becker, Marie Adams, Daniel Newhouse, et al.in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250, pS129 α-synuclein (81A, Biolegend 825701, 1:1000). For immunohistochemistry,

(2) CD11c(+) microglia promote white matter repair after ischemic strokeCell death & diseaseFebruary 24, 2023Jia J, Zheng L, Ye L, Chen J, Shu S, Xu S, Bao X, Xia S, Liu R, Xu Y, et al.#ab40390; Anti-NeuN Antibody, 1:500, Abcam, #ab177487; Anti-NF-H Antibody (SMI32), 1:500, BioLegend, #801702; Anti-Pdgfrα Antibody, 1:300, BD Biosciences, #559774; Anti-TMEM119, 1:500, Synaptic Systems, #400011.

(3) Insulin-like Growth Factor-1 Prevents Hypoxia/Reoxygenation-Induced White Matter Injury in Sickle Cell MiceBiomedicinesFebruary 24, 2023Hazra R, Hubert H, Little-Ihrig L, Ghosh S, Ofori-Acquah S, Hu X, Novelli EM.antibodies for non-phosphorylated anti-neurofilament H (SMI32, Biolegend, San Diego, CA, USA, cat# 801702) and myelin basic protein (MBP, Proteintech, Rosemont, IL, USA, cat# 1048-1-AP), two markers of white

(4) Efficient generation of lower induced motor neurons by coupling Ngn2 expression with developmental cuesCell reportsJanuary 31, 2023Limone F, Guerra San Juan I, Mitchell JM, Smith JLM, Raghunathan K, Meyer D, Ghosh SD, Couto A, Klim JR, Joseph BJ, et al.RRID:AB_2200400" "Mouse monoclonal anti-Neurofilament-H, nonphosphorylated (SMI-32)",BioLegend,"Cat# 801702, RRID:AB_2715852" Goat polyclonal anti-Choline Acetyltransferase,Millipore,Cat# AB144P; RRID:AB_11212924 Rabbit

(5) A reference human induced pluripotent stem cell line for large-scale collaborative studiesCell stem cellDecember 1, 2022Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, et al.,Sigma Aldrich,T2200; RRID:AB_262133 Mouse anti-HB9,DSHB,81.5c10; RRID:AB_2145209 Mouse anti-SMI32,Biolegend,"801702, RRID: AB_2715852" Mouse anti-Islet 1/2,DSHB,39.4D5; RRID:AB_2314683 Rabbit anti-NF-H,Millipore,ab1989;

(6) Selenoprotein I deficiency in T cells promotes differentiation into tolerant phenotypes while decreasing Th17 pathologyJournal of Leukocyte BiologyNovember 25, 2022Chi Ma, FuKun W. Hoffmann, Lance G. Nunes, Frank Urena, Anastasia Andrukhiv, Mariana Gerschenson, Matthew W. Pitts, Peter R. HoffmannFor immunohistochemistry, antibodies included rabbit polyclonal anti-CD3 (IS503, 1:200; Dako) and SMI-32 Ab (801702, 1:5000; BioLegend). For immunofluorescence, PE-anti-IL-17A was used (17B7, 1:500; Ebioscience). Myelin

(7) Distribution of prototypical primary cilia markers in subtypes of retinal ganglion cellsThe Journal of comparative neurologyAugust 1, 2022Kowal TJ, Dhande OS, Wang B, Wang Q, Ning K, Liu W, Berbari NF, Hu Y, Sun Y.neurofilament H,"a-RGCAON-S and AOFF-T alpha-like RGCs, but not AOFF-S",Straznicky 1992,Antibody: Biolegend 801702,<,+ OPN,Osteopontin,a-RGC,Duan 2015,Antibody: DHSB MPIIIB10(1)RRID: AB_2286610,<,+ CB2,Calretinin,Transient

(8) Moxifloxacin rescues SMA phenotypes in patient-derived cells and animal modelCellular and Molecular Life SciencesAugust 1, 2022Camille Januel, Giovanna Menduti, Kamel Mamchaoui, Cecile Martinat, Ruben Artero, Piotr Konieczny, Marina Boido488,A-21208,Thermo Fisher Scientific,1:1000 SMA delta 7 mice,Primary,Neurofilament H (NF-H) SMI32,801702,Mouse,BioLegend,1:1000 SMA delta 7 mice,Primary,Cleaved Caspase 3 (Asp175),9661,Rabbit,Cell Signaling Technology

(9) Differences in spike generation instead of synaptic inputs determine the feature selectivity of two retinal cell typesNeuronJuly 6, 2022Wienbar S, Schwartz GW.RESOURCE,SOURCE,IDENTIFIER Antibodies/ Probes,Antibodies/ Probes,Antibodies/ Probes Mouse anti-SMI32,BioLegend,"801702, RRID AB_2715852" Goat anti-ChAT,Millipore,"AB144P, RRID AB_2079751" Alexa 568 anti-mouse,Life Technologies,"A-11004,

(10) GDAP1 loss of function inhibits the mitochondrial pyruvate dehydrogenase complex by altering the actin cytoskeletonCommunications biologyJune 3, 2022Wolf C, Pouya A, Bitar S, Pfeiffer A, Bueno D, Rojas-Charry L, Arndt S, Gomez-Zepeda D, Tenzer S, Bello FD, et al.14-9843-82), anti-MAP2 (1:500, Synaptic Systems, 188004), anti-Smi32 (Clone SMI32, 1:1000, Biolegend 801702), anti-beta-III tubulin (1:1000, R&D Systems, MAB1195) were treated in 1X Roti®-Block at 4 °C overnight.

(11) Differential effects of experimental glaucoma on intrinsically photosensitive retinal ganglion cells in miceJournal of Comparative NeurologyJune 1, 2022Jingyi Gao, Erin M. Griner, Mingna Liu, Joanna Moy, Ignacio Provencio, Xiaorong Liuincluded a rabbit polyclonal anti-melanopsin (1:2000) (Panda et al., 2002), mouse anti-SMI-32 (Biolegend, 801702/01, 1:250, RRID: AB_2715852), mouse anti-Brn3a (Millipore, MAB1585, 1:125, RRID: AB_94166) and rabbit

(12) Morphological and distributional properties of SMI‐32 immunoreactive ganglion cells in the rat retinaJournal of Comparative NeurologyJune 1, 2022Huiying Tan, Xiaotao Li, Kang Huang, Moxuan Luo, Liping Wangserum for 1 h at room temperature. Retinas were then incubated in anti‐SMI‐32 antibody (Biolegend, Cat# 801702, Mouse, 1:1000, research resource identifiers (RRID): AB_2715852) overnight at 4°C. Following three

(13) Optimization of Long-Term Human iPSC-Derived Spinal Motor Neuron Culture Using a Dendritic Polyglycerol Amine-Based SubstrateASN NeuroJanuary 13, 2022Louise Thiry, Jean-Pierre Clément, Rainer Haag, Timothy E Kennedy, Stefano Stifanianti-non-phosphorylated neurofilament H antibody SMI32 (1/100; BioLegend; San Diego, CA, USA; Cat. No. 801702). The indicated DSHB monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank,

(14) Alpha retinal ganglion cells in pigmented mice retina: number and distributionFrontiers in neuroanatomyJanuary 1, 2022Gallego-Ortega A, Norte-Muñoz M, Di Pierdomenico J, Avilés-Trigueros M, de la Villa P, Valiente-Soriano FJ, Vidal-Sanz M.,214 005 Synaptic Systems,1:500 ,"Mouse anti Neurofilament H (NF-H), Nonphosphorylated (SMI32)",BioLegend 801702,"1:2,000" ,Rabbit anti OPN4,AB-N39 Advanced Targeting Systems,"1:1,000" ,Rabbit anti RBPMS,Invitrogen

(15) Differential NPY-Y1 Receptor Density in the Motor Cortex of ALS Patients and Familial Model of ALSBrain sciencesJuly 23, 2021Clark CM, Clark RM, Hoyle JA, Chuckowree JA, McLean CA, Dickson TC.

(16) Treg cell-derived osteopontin promotes microglia-mediated white matter repair after ischemic strokeImmunityJuly 13, 2021Shi L, Sun Z, Su W, Xu F, Xie D, Zhang Q, Dai X, Iyer K, Hitchens TK, Foley LM, et al.

(17) Inhibition of CD38 and supplementation of nicotinamide riboside ameliorate lipopolysaccharide-induced microglial and astrocytic neuroinflammation by increasing NAD⁺Journal of neurochemistryJuly 1, 2021Roboon J, Hattori T, Ishii H, Takarada-Iemata M, Nguyen DT, Heer CD, O'Meally D, Brenner C, Yamamoto Y, Okamoto H, et al.

(18) OFF-transient alpha RGCs mediate looming triggered innate defensive responseCurrent biology : CBJune 7, 2021Wang F, Li E, De L, Wu Q, Zhang Y.

(19) Heterochrony of postnatal accumulation of nonphosphorylated heavy-chain neurofilament by neurons of the cat dorsal lateral geniculate nucleusThe Journal of comparative neurologyMay 1, 2021Mikhalkin A, Nikitina N, Merkulyeva N.

(20) Selected Clostridia Strains from The Human Microbiota and their Metabolite, Butyrate, Improve Experimental Autoimmune EncephalomyelitisNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsApril 1, 2021Calvo-Barreiro L, Eixarch H, Cornejo T, Costa C, Castillo M, Mestre L, Guaza C, Martínez-Cuesta MDC, Tanoue T, Honda K, et al.

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(1)

2/26/2020Good NF-H Antibody from BioLegend T SenThe product is reliable and consistent and good quality.

Purified (azide-free) anti-Neurofilament H (NF-H), Nonphosphorylated from BioLegend

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Purified (azide-free) anti-Neurofilament H (NF-H), Nonphosphorylated from BioLegend

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