Fig 2: DYNLRB1 ensures spindle pole integrity in mitosis.a Tissue and cell-type-specific expression of the indicated mouse genes. b The quantification of the Dynlrb1 mRNA expression level normalized to the average value of the controls in B16-F1 cells. The mean values with SD of three independent experiments are shown. c The quantification of mitotic cell population in B16-F1 cells. The mean values with SD of three independent experiments are shown (n = 2275 and 2058 for control and Dynlrb1 RNAi, respectively). d Immunostaining of B16-F1 cells in metaphase and the number of spindle poles. Mean values with SD from three independent experiments for both conditions are shown (n = 65 for both conditions). Scale bar: 5 µm. e Quantification of chromosome alignment and spindle defects in B16-F1 cells. The mean values with SD of three independent experiments are shown (n = 90 and 209 for control and Dynlrb1 RNAi, respectively). f Immunostaining of B16-F1 cells. Scale bar: 10 µm. g Immunostaining of B16-F1 cells in metaphase and quantification of CETN2 foci number from three independent experiments for both conditions (n = 43 for both conditions). Scale bar: 5 µm (0.5 µm, magnified panel). h The quantification of PCNT foci accompanying CETN2 foci in B16-F1 metaphase cells. The mean values with SD of three independent experiments are shown. PCNT foci (n = 190, 104, and 196) were pooled from 95, 49, and 46 cells for control, Dynlrb1 RNAi (bipolar), and Dynlrb1 RNAi (multipolar), respectively. i Immunostaining of B16-F1 cells in metaphase. The graphs show the line scan analysis of NuMA intensity between each spindle pole. Scale bar: 5 µm. j The quantification of NuMA signal intensity at metaphase spindle poles in B16-F1 cells normalized to the average value of the controls. The mean values with SD from three independent experiments are shown (n = 118 and 138 for control and Dynlrb1 RNAi, respectively). Only cells that formed bipolar spindles were quantified. Two-tailed t-tests were used in (b–d), (e), (g), and (j). One-way ANOVA with Dunnett’s multiple comparisons test was used in (h). ns not significant, **p < 0.01, ***p < 0.001, ****p < 0.0001.