Fig 1: Effect of transection of the sciatic nerve on expression of the unfolded protein response (UPR) in skeletal muscle. (A) Representative images of the control and denervated TA muscles immunostained against XBP1 and DAPI. Scale bar = 100 µm. (B) Average percentage of nuclei expressing XBP1 in the control and denervated TA muscles. (C) Representative images of the control and denervated TA muscles immunostained against ATF6 and DAPI. (D) Representative images of the control and denervated TA muscles immunostained against ATF4 and DAPI. * p < 0.05, values significantly different from the control mice by a paired two-tailed t-test.
Fig 2: Effect of transection of the sciatic nerve on expression of the unfolded protein response (UPR) in the neuromuscular junction (NMJ). (A) Representative images of the control and denervated GAS muscles immunostained against XBP1 and with a snake toxin that has a high affinity for AChRs (a-bungarotoxin-tetramethylrhodamine). Scale bar = 100 µm. (B) Average percentage of NMJ expressing XBP1 in control and denervated muscle. N = 6/group. (C) Representative images of the control and denervated GAS muscles immunostained against ATF6 and with a snake toxin that has a high affinity for AChRs (a-bungarotoxin-tetramethylrhodamine). Scale bar = 100 µm. (D) Representative images of the control and denervated GAS muscles immunostained against ATF4 and with a snake toxin that has a high affinity for AChRs (a-bungarotoxin-tetramethylrhodamine). Scale bar = 100 µm. * p < 0.05, values significantly different from the control mice by a paired two-tailed t-test.
Supplier Page from Thermo Fisher Scientific for ATF4 Antibody