Fig 1: BioIDs of Mrpl4 and Cbp3 and generation of proximity interactome network(A) Western blots showing successful tagging of Mrpl4 and Kgd4 with BirA* by immunodecorating with anti-Mrpl4 and anti-Kgd4 antibody, respectively. Immunodecoration with anti-Cox2 shows that mitochondrial translation is not impacted in the BirA*-tagged cell lines.(B) During purification of biotinylated proteins, instead of tryptic digestion, SDS-loading dye was added to the beads and boiled at 95°C for 20 min under shaking to elute the proteins. The lysate (T), unbound fraction (U), and elutions (E) were separated by SDS-PAGE followed by western blotting.(C and D) Volcano plots of Mrpl4 and Cbp3 individual BioIDs.(E) The enrichment data obtained from (C) and (D) were used to create a bait-prey network in Cytoscape; each protein is shown as a node, connect by red lines (edges) that are quantitatively color-mapped. *, cross-reactive bands; iL, long isoform of Kgd4; iS, short isoform of Kgd4.