Fig 1: The expression of the pro-SP-C, HOPX and vimentin proteins in the mouse lungs at different phases of injury following irradiation. The relative levels of the proSP-C, HOPX and vimentin proteins were examined by western blot (A). Graphs (B), (C) and (D) show the relative levels of the three phenotype markers after exposure to IR. The relative amounts of proSP-C, HOPX and vimentin in the graphs were obtained by the ratios of the target protein pixels to the corresponding ß-actin pixels. The data presented are shown as the mean ± SD. Statistical analyses between groups were performed using Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. h, hour; w, week; m, month. Note: The proSP-C and vimentin proteins were detected on the same blot membranes, and the western blots for HOPX were performed separately.
Fig 2: The merged images of the coexpression of proSP-C (AEC II cell marker) with EpCAM (epithelial stem cell marker) (A), HOPX (AEC I cell marker) (B), or vimentin (mesenchymal cell marker) (C) in the lungs of mice treated with IR and control-treated mice. Double immunofluorescence staining was used to detect protein expression. In the representative photomicrographs, the color of the pro-SP-C protein is red, the EpCAM, HOPX and vimentin proteins are green, and the nuclei are blue. The yellow fluorescence in AEC II is due to the overlap of red and green (arrows). Coexpression of EpCAM/proSP-C, HOPX/proSP-C or vimentin/proSP-C in the lung tissues was used to track AEC II dynamic phenotypes at different phases of injury after irradiation. Magnification: 400x. h, hour; w, week; m, month.
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