Fig 1: Curcumin reduces ERRα expression and Cholesterol content in ACC cells. (A–F) ACC cells were treated with the indicated doses of curcumin for 24 h and ERRα mRNA and protein expression were assessed by real-time RT-PCR (A,C,E) and Western blotting analysis (B,D,F). (G,I) Colorimetric assay was used to measure cholesterol extracted from H295R cells treated for 24h as follows: (G) curcumin (0, 20, 40 μM) and (I) vehicle (veh, DMSO), Mitotane (Mit, 10 μM), curcumin (Cur 40 μM) and their combination (Mit + Cur). (H) Western blotting of HMGCR and SR-BI in curcumin-treated H295R cells. (J) H295R cells were untreated (basal, BS), treated with vehicle (veh, DMSO), Mitotane (Mit, 10 μM), curcumin (Cur 40 μM) and their combination (Mit + Cur) 48 h. Cell viability was evaluated by MTT assay. Data and images are from at least three independent experiments. (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 vs. 0 µM; ns: not significant).