Fig 1: Repression of BNIP3 prevents cell injury induced by miR-103 downregulation. (a) Relative expression of BNIP3 was detected by western blot after treatment with H2O2. Data are shown as mean ± SD, *P < 0.05, *compared with the control group. (b) Relative expression of BNIP3 was detected by western blot after treatment with 24 h for bafA1. Data are shown as mean ± SD, *P < 0.05, *P < 0.05, #P < 0.05, *compared with the control group, #compared with the H2O2 group. (c) Relative expression of BNIP3 was detected by western blot after treatment with pre-103. Data are shown as mean ± SD, *P < 0.05, #P < 0.05, ?P < 0.05, *compared with the NC group, #compared with the H2O2 group, ?compared with the pre-103+H2O2 group. (d) Relative expression of BNIP3 was detected by western blot after treatment with Inh-103. Data are shown as mean ± SD, *P < 0.05, #P < 0.05, *compared with the NC group, #compared with the H2O2 group. (e) Relative expression of BNIP3 was detected by western blot after treatment with, NC, siBNIP3, Inh-103, Inh-103+siBNIP3. Data are shown as mean ± SD, **P < 0.01, ##P < 0.01, *compared with the NC group, #compared with the Inh-103 group. (f) Cell viability was studied by MTS assay, **P < 0.01, *P < 0.05, #P < 0.05, ?P < 0.05, *compared with the NC group, #compared with the H2O2 group, ?compared with the Inh-103+H2O2 group. (g, h) Relative expression of autophagy proteins was detected by western blot. Data are shown as mean ± SD, *P < 0.05, #P < 0.05, ?P < 0.05, *compared with the H2O2 group, #compared with the SiBNIP3+H2O2 group, ?compared with the Inh-103+H2O2 group.
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