Fig 1: Confocal images and 3D reconstruction of uninfected and RSV-infected HAE cultures. Legend: Immunofluorescence confocal microscopy images of an uninfected adult HAE culture stained for ciliated (A) and goblet cells (B). Confocal image of an RSV-infected adult HAE culture after 120 h of RSV infection (C). The 3D illustrations of an uninfected HAE culture (D,E); 3D illustration of an RSV-infected HAE culture (F). Blue: cell nuclei (DAPI staining), red: ciliated cells (staining with anti-b tubulin antibody), magenta: goblet cells (staining with anti-MUC5AC), green: RSV infection, primarily visible in ciliated cells (staining with polyclonal anti-RSV antibodies).
Fig 2: (A) qPCR quantification of mucin (MUC5AC) and dipeptidyl peptidase-4 (DDP-IV) with undifferentiated HT-29 cells used as the control, #p < 0.05. (B) Confocal micrograph of co-cultured Caco-2 and HT-29/MTX cells. Blue = cell nuclei (DAPI); green = anti-MUC5AC. Scale bar = 50 µm.
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