Anti-CRISPR-Cas9 antibody [EPR18991] ab189380 from Abcam

Product Specs
ItemAnti-CRISPR-Cas9 antibody [EPR18991]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab189380
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
HostRabbit
Antibody TypePrimary
NCBI Gene AliasesSPy1046
ClonalityMonoclonal
CloneEPR18991
Gene Namecas9
ReactivityStreptococcus pyogenes
TargetCRISPR-Cas9

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Structure-guided inhibition of the cancer DNA-mutating enzyme APOBEC3ANature CommunicationsOctober 11, 2023Stefan Harjes, Harikrishnan M. Kurup, Amanda E. Rieffer, Maitsetseg Bayarjargal, Jana Filitcheva, Yongdong Su, Tracy K. Hale, Vyacheslav V. Filichev, Elena Harjes, Reuben S. Harris, et al.(#T5168, 1:10000, Sigma-Aldrich), rabbit anti-HA (#3724 S, 1:2500, Cell Signaling), and rabbit anti-Cas9 (#ab189380, 1:5000, Abcam). Secondary antibodies used were goat anti-rabbit IRdye800 (LI-COR, #925-32211, 1:10000)

(2) APOBEC Reporter Systems for Evaluating diNucleotide Editing LevelsThe CRISPR JournalOctober 1, 2023Amanda E. Rieffer, Yanjun Chen, Daniel J. Salamango, Sofia N. Moraes, Reuben S. Harrisincubation was conducted as previously reported.42 Primary antibodies used include rabbit anti-Cas9 (No. ab189380, 1:5000; Abcam), mouse anti-tubulin (No. T5168, 1:10,000; Sigma-Aldrich), and rabbit anti-FLAG (No.

(3) Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapyNature communicationsJuly 11, 2023Liu X, Zhuang Y, Huang W, Wu Z, Chen Y, Shan Q, Zhang Y, Wu Z, Ding X, Qiu Z, et al.dilution, Abcam, Cat. #ab18256, RRID:AB_444360), anti-SpCas9 antibody (1:10000 dilution, Abcam, Cat. #ab189380, Clone: EPR18991, RRID:), anti-β-Actin antibody (1:1000 dilution, CST, Cat. #3700, Clone: 8H10D10, RRID:

(4) Structure-guided inhibition of the cancer DNA-mutating enzyme APOBEC3AbioRxivJune 21, 2023Stefan Harjes, Harikrishnan M. Kurup, Amanda E. Rieffer, Maitsetseg Bayarjargal, Jana Filitcheva, Yongdong Su, Tracy K. Hale, Vyacheslav V. Filichev, Elena Harjes, Reuben S. Harris, et al.(#T5168, 1:10000, Sigma-Aldrich), rabbit anti-HA (#3724S, 1:2500, Cell Signaling), and rabbit anti-Cas9 (#ab189380, 1:5000, Abcam). Secondary antibodies used were goat anti-rabbit IRdye800 (LI-COR, #925-32211, 1:10000)

(5) A molecular glue approach to control the half-life of CRISPR-based technologiesbioRxivMarch 24, 2023Vedagopuram Sreekanth, Max Jan, Kevin T. Zhao, Donghyun Lim, Jessie R. Davis, Marie McConkey, Veronica Kovalcik, Sam Barkal, Benjamin K. Law, James Fife, et al.PVDF membranes were subjected to immunoblotting with a monoclonal antibody anti-Cas9 antibody (Abcam: ab189380 or ab191468), anti-FLAG antibody (Cell Signaling Technologies: 2368S), and a monoclonal anti-β-actin

(6) Lung-selective mRNA delivery of synthetic lipid nanoparticles for the treatment of pulmonary lymphangioleiomyomatosisProceedings of the National Academy of SciencesFebruary 22, 2022Min Qiu, Yan Tang, Jinjin Chen, Rachel Muriph, Zhongfeng Ye, Changfeng Huang, Jason Evans, Elizabeth P. Henske, Qiaobing Xuskimmed milk for 1 h at room temperature, and incubated with anti–CRISPR-Cas9 primary antibody (Abcam, ab189380) overnight at 4 °C. The membrane was washed with TBST (Tris-buffered saline, 0.1% Tween 20) five times,

(7) CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathwayProtein & cellMay 1, 2020Xu S, Kim J, Tang Q, Chen Q, Liu J, Xu Y, Fu X.(9009S) Anti-CHK2,Cell signaling (6334S) Anti-pCHK2,Cell signaling (2197S) Anti-CAS9,"Cell signaling (14697S), Abcam (ab189380), Novus Biologicals (NBP2-52717)" ,(NBP2-52717) Anti-β-actin,Cell signaling (4970S) Anti-Flag,Thermo Fisher

(8) MagnEdit—interacting factors that recruit DNA-editing enzymes to single base targetsLife Science AllianceApril 1, 2020Jennifer L McCann, Daniel J Salamango, Emily K Law, William L Brown, Reuben S Harriswith an LI-COR Odyssey instrument. Primary antibodies used in these experiments were rabbit anti-Cas9 (ab189380; Abcam), mouse anti-tubulin (T5168; Sigma-Aldrich), rabbit anti-HA (3724S; Cell Signaling), and mouse

(9) A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9Nature CommunicationsDecember 1, 2018Lun Cui, Antoine Vigouroux, François Rousset, Hugo Varet, Varun Khanna, David Bikardpolyvinylidene difluoride membranes at 15 volts overnight. Rabbit monoclonal antibodies to SpCas9 (ab189380, Abcam, diluted 10,000-fold) and rabbit polyclonal antibody to RecA (ab63797, Abcam, diluted 3,000-fold)

Be the First to Write a Review!

Anti-CRISPR-Cas9 antibody [EPR18991] from Abcam

Abcam

Citations (9)

Reviews

Anti-CRISPR-Cas9 antibody [EPR18991] from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map