Fig 1: circITGB1 is highly enriched in EVs from the plasma of AMI patients. (a) Representative micrograph of EVs isolated from plasma of healthy control and AMI patients, as detected by TEM (n = 3). (b, c) Flow cytometry analysis of microspheres coated with EV-rich fractions isolated from plasma of healthy controls identified the presence of exosomal markers CD63, CD81, and HSP70, both at baseline (control, blue) and AMI (pink). Yellow curve indicates EV-coated microspheres stained with secondary antibodies (control). Collective analyses of FACS results in the two groups, n = 3. (d) NTA analysis of EVs derived from control and AMI plasma. (e) Western blots confirmed the presence of exosomal marker proteins CD63, CD81, and HSP70 in healthy control and AMI samples, n = 3. Levels of all proteins were increased in AMI. (f) Levels of circITGB1 in the EVs and supernatant fractions (Sup) of the plasma from patients with AMI and healthy controls, as detected by qPCR (n = 10 for each group); GAPDH was measured as a reference gene. Data are expressed as means ± SD. Statistical significance was calculated using Student's t-test or one-way ANOVA. **P < 0.01 compared with the control group.